Detailed information of TA system TA178430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3175526..3176164
• Replicon: chromosome
• Accession: NZ_CP063483
• Organism: Escherichia coli strain EM03-18-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: IQS21_RS15285
• Protein ID: WP_001447010.1
• Coordinates: 3175988..3176164 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IQS21_RS15280
• Protein ID: WP_001270286.1
• Coordinates: 3175526..3175942 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS21_RS15260	3170678..3171619	-	942	WP_001251304.1	ABC transporter permease	-
IQS21_RS15265	3171620..3172633	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
IQS21_RS15270	3172651..3173796	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
IQS21_RS15275	3174041..3175447	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
IQS21_RS15280	3175526..3175942	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IQS21_RS15285	3175988..3176164	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IQS21_RS15290	3176386..3176616	+	231	WP_000494244.1	YncJ family protein	-
IQS21_RS15295	3176708..3178669	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IQS21_RS15300	3178742..3179278	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
IQS21_RS15305	3179331..3180545	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T178430 WP_001447010.1 NZ_CP063483:c3176164-3175988 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T178430 NZ_CP084023:c1556008-1555688 [Pectobacterium aroidearum]
ATGCAAACAACACCCGCAATCCCGCCACGGGAGGATAACCCCTGTGCGTCGCACATAGCAGTCTGGCAGCAACTTCTGAC
CTACCTGCTGGAAAAACACTACGGACTCTCGCTGAATGACACACCGTTCTGCGAGGAAAATGTGATTCAGGAGCATATCG
ATGCTGGCATCACGTTGCTCAATGCCGTCAATTTTCTGGTGGAAAAATACGAACTGGTACGCATTGATCGCAACGGTTTT
AACTGGCAGGAGCAATCACCGTTTATCACGACTGTCGATATCCTTCGCGCCAGACGTGCGGCGGGATTACTTAAGGCATA
A

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT178430 WP_001270286.1 NZ_CP063483:c3175942-3175526 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT178430 NZ_CP084023:c1556413-1556078 [Pectobacterium aroidearum]
ATGTCATCGTCTGACACCCCGGAATGGGGACTAAAATGTGCCATCACGCCGCGATTCGGAGCCAGACTGGTGCAGGAAGG
TAACCGTCTGCATTATCTGGTAGACCGGGCCAGCTTTATCGGTACTTTTAGCAAGGCAGAAGCCCGTAATCTGGAACACG
TCTTCCCTGAGTTGATTAAGCAGTTGGAACAAAAACTGAGAACGGGGGAACTGAATCCCCATCAGCAGGGCTGCGTCACG
CTACATTGCACCGAATGGACCTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTGTACATCGTTATTTACCCCTCTTC
TGCGGCAGCAGAATAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8