Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1552778..1553432 Replicon chromosome
Accession NZ_CP063483
Organism Escherichia coli strain EM03-18-08

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IQS21_RS07355 Protein ID WP_000244777.1
Coordinates 1553025..1553432 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS21_RS07350 Protein ID WP_000354046.1
Coordinates 1552778..1553044 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS21_RS07325 1547947..1548690 + 744 WP_000951964.1 SDR family oxidoreductase -
IQS21_RS07330 1548747..1550180 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
IQS21_RS07335 1550225..1550536 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IQS21_RS07340 1550700..1551359 + 660 WP_000250274.1 hemolysin III family protein -
IQS21_RS07345 1551555..1552535 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IQS21_RS07350 1552778..1553044 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS21_RS07355 1553025..1553432 + 408 WP_000244777.1 toxin CptA Toxin
IQS21_RS07360 1553472..1553993 - 522 WP_001055874.1 flavodoxin FldB -
IQS21_RS07365 1554105..1555001 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IQS21_RS07370 1555026..1555736 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS21_RS07375 1555742..1557475 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T178416 WP_000244777.1 NZ_CP063483:1553025-1553432 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178416 NZ_CP084008:c1975536-1975174 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178416 WP_000354046.1 NZ_CP063483:1552778-1553044 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178416 NZ_CP084008:c1975703-1975533 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References