Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4483462..4484116 Replicon chromosome
Accession NZ_CP063479
Organism Escherichia coli strain EM06-18-14

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag IQS22_RS21490 Protein ID WP_000244772.1
Coordinates 4483462..4483869 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS22_RS21495 Protein ID WP_000354046.1
Coordinates 4483850..4484116 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS22_RS21470 4479419..4481152 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
IQS22_RS21475 4481158..4481868 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS22_RS21480 4481893..4482789 - 897 WP_000806637.1 site-specific tyrosine recombinase XerD -
IQS22_RS21485 4482901..4483422 + 522 WP_001055874.1 flavodoxin FldB -
IQS22_RS21490 4483462..4483869 - 408 WP_000244772.1 protein YgfX Toxin
IQS22_RS21495 4483850..4484116 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS22_RS21500 4484359..4485339 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
IQS22_RS21505 4485535..4486194 - 660 WP_000250274.1 hemolysin III family protein -
IQS22_RS21510 4486358..4486669 - 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
IQS22_RS21515 4486714..4488147 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
IQS22_RS21520 4488204..4488947 - 744 WP_000951941.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T178400 WP_000244772.1 NZ_CP063479:c4483869-4483462 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178400 NZ_CP083992:c208002-207532 [Escherichia coli]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178400 WP_000354046.1 NZ_CP063479:c4484116-4483850 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178400 NZ_CP083992:c208442-207999 [Escherichia coli]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References