Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4022823..4023448 Replicon chromosome
Accession NZ_CP063479
Organism Escherichia coli strain EM06-18-14

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag IQS22_RS19270 Protein ID WP_000911329.1
Coordinates 4022823..4023221 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag IQS22_RS19275 Protein ID WP_000450524.1
Coordinates 4023221..4023448 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS22_RS19250 4018701..4018901 + 201 WP_000383836.1 YpfN family protein -
IQS22_RS19255 4019011..4019709 - 699 WP_000679823.1 esterase -
IQS22_RS19260 4019783..4021798 - 2016 WP_000829295.1 tRNA cytosine(34) acetyltransferase TmcA -
IQS22_RS19265 4021813..4022676 - 864 WP_001267498.1 neutral zinc metallopeptidase -
IQS22_RS19270 4022823..4023221 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
IQS22_RS19275 4023221..4023448 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
IQS22_RS19280 4023603..4024316 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
IQS22_RS19285 4024529..4025563 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
IQS22_RS19290 4025580..4026458 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
IQS22_RS19295 4026604..4027176 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
IQS22_RS19300 4027176..4027646 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T178398 WP_000911329.1 NZ_CP063479:c4023221-4022823 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T178398 NZ_CP083992:c24247-23804 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT178398 WP_000450524.1 NZ_CP063479:c4023448-4023221 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT178398 NZ_CP083992:c24474-24244 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References