Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1189896..1190731 Replicon chromosome
Accession NZ_CP063479
Organism Escherichia coli strain EM06-18-14

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2S4A272
Locus tag IQS22_RS05720 Protein ID WP_001094443.1
Coordinates 1189896..1190273 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7LDN9
Locus tag IQS22_RS05725 Protein ID WP_001285607.1
Coordinates 1190363..1190731 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS22_RS05695 1185566..1187035 + 1470 WP_001022619.1 serine/threonine protein kinase -
IQS22_RS05700 1187236..1188501 - 1266 WP_001218329.1 integrase arm-type DNA-binding domain-containing protein -
IQS22_RS05705 1188963..1189109 - 147 WP_001290178.1 hypothetical protein -
IQS22_RS05710 1189194..1189391 - 198 WP_001327226.1 DUF957 domain-containing protein -
IQS22_RS05715 1189411..1189899 - 489 WP_000761685.1 hypothetical protein -
IQS22_RS05720 1189896..1190273 - 378 WP_001094443.1 TA system toxin CbtA family protein Toxin
IQS22_RS05725 1190363..1190731 - 369 WP_001285607.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
IQS22_RS05730 1190811..1191032 - 222 WP_000692350.1 DUF987 domain-containing protein -
IQS22_RS05735 1191119..1191595 - 477 WP_001186165.1 RadC family protein -
IQS22_RS05740 1191610..1192095 - 486 WP_000206664.1 antirestriction protein -
IQS22_RS05745 1192187..1193005 - 819 WP_001175163.1 DUF945 domain-containing protein -
IQS22_RS05750 1193095..1193328 - 234 WP_001278287.1 DUF905 family protein -
IQS22_RS05755 1193334..1194011 - 678 WP_001097301.1 hypothetical protein -
IQS22_RS05760 1194159..1194839 - 681 WP_001282919.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island tet(B) papD / papC / fimD / fimF / fimG / fimH 1133491..1227391 93900


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14058.97 Da        Isoelectric Point: 7.3523

>T178383 WP_001094443.1 NZ_CP063479:c1190273-1189896 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T178383 NZ_CP083990:1956288-1956391 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13689.53 Da        Isoelectric Point: 6.4669

>AT178383 WP_001285607.1 NZ_CP063479:c1190731-1190363 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT178383 NZ_CP083990:c1956429-1956158 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S4A272

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0JLU8

References