Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 183489..184120 Replicon chromosome
Accession NZ_CP063479
Organism Escherichia coli strain EM06-18-14

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag IQS22_RS00990 Protein ID WP_001259388.1
Coordinates 183845..184120 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag IQS22_RS00985 Protein ID WP_000593555.1
Coordinates 183489..183848 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS22_RS00960 179621..180565 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
IQS22_RS00965 180562..181395 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
IQS22_RS00970 181395..182159 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
IQS22_RS00975 182156..182962 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
IQS22_RS00980 182968..183369 + 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
IQS22_RS00985 183489..183848 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IQS22_RS00990 183845..184120 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
IQS22_RS00995 184193..185317 - 1125 WP_001615305.1 ABC transporter permease -
IQS22_RS01000 185317..188052 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
IQS22_RS01005 188049..189116 - 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T178378 WP_001259388.1 NZ_CP063479:c184120-183845 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T178378 NZ_CP083990:c1108350-1108021 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT178378 WP_000593555.1 NZ_CP063479:c183848-183489 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT178378 NZ_CP083990:c1108688-1108371 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References