Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4665181..4665835 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag IQS23_RS22295 Protein ID WP_000244765.1
Coordinates 4665428..4665835 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS23_RS22290 Protein ID WP_000354046.1
Coordinates 4665181..4665447 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS22270 4661269..4662702 - 1434 WP_141031856.1 6-phospho-beta-glucosidase BglA -
IQS23_RS22275 4662747..4663058 + 312 WP_001182951.1 N(4)-acetylcytidine aminohydrolase -
IQS23_RS22280 4663222..4663881 + 660 WP_000250274.1 hemolysin III family protein -
IQS23_RS22285 4663958..4664938 - 981 WP_086518704.1 tRNA-modifying protein YgfZ -
IQS23_RS22290 4665181..4665447 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS23_RS22295 4665428..4665835 + 408 WP_000244765.1 protein YgfX Toxin
IQS23_RS22300 4665875..4666396 - 522 WP_001055874.1 flavodoxin FldB -
IQS23_RS22305 4666508..4667404 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IQS23_RS22310 4667429..4668139 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS23_RS22315 4668145..4669878 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T178375 WP_000244765.1 NZ_CP063476:4665428-4665835 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178375 NZ_CP083990:717224-717520 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178375 WP_000354046.1 NZ_CP063476:4665181-4665447 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178375 NZ_CP083990:717522-717917 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References