Detailed information of TA system TA178374

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4543616..4544448
• Replicon: chromosome
• Accession: NZ_CP063476
• Organism: Escherichia coli strain EM10-18-28

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2VCF5
• Locus tag: IQS23_RS21770
• Protein ID: WP_000854681.1
• Coordinates: 4543616..4543993 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IQS23_RS21775
• Protein ID: WP_088474659.1
• Coordinates: 4544083..4544448 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS23_RS21740	4538744..4539892	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
IQS23_RS21745	4539964..4540947	-	984	WP_032152721.1	KpsF/GutQ family sugar-phosphate isomerase	-
IQS23_RS21750	4541758..4541928	-	171	Protein_4247	IS110 family transposase	-
IQS23_RS21755	4542270..4543112	-	843	WP_001529559.1	DUF4942 domain-containing protein	-
IQS23_RS21760	4543197..4543394	-	198	WP_023563519.1	DUF957 domain-containing protein	-
IQS23_RS21765	4543470..4543619	-	150	Protein_4250	hypothetical protein	-
IQS23_RS21770	4543616..4543993	-	378	WP_000854681.1	TA system toxin CbtA family protein	Toxin
IQS23_RS21775	4544083..4544448	-	366	WP_088474659.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IQS23_RS21780	4544526..4544747	-	222	WP_000692300.1	DUF987 domain-containing protein	-
IQS23_RS21785	4544810..4545286	-	477	WP_001186171.1	RadC family protein	-
IQS23_RS21790	4545301..4545786	-	486	WP_000206670.1	antirestriction protein	-
IQS23_RS21795	4545878..4546696	-	819	WP_001175163.1	DUF945 domain-containing protein	-
IQS23_RS21800	4546786..4547019	-	234	WP_073506913.1	DUF905 family protein	-
IQS23_RS21805	4547025..4547702	-	678	WP_001097301.1	hypothetical protein	-
IQS23_RS21810	4547850..4548530	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-116)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13963.89 Da
• Isoelectric Point: 7.4348

>T178374 WP_000854681.1 NZ_CP063476:c4543993-4543616 [Escherichia coli]
MKTLPDTHIREASHCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTSHNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T178374 NZ_CP083990:651314-651628 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13554.36 Da
• Isoelectric Point: 6.2034

>AT178374 WP_088474659.1 NZ_CP063476:c4544448-4544083 [Escherichia coli]
VSDTLPGTTYPDNNNRPWWGLPCTVMPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSGE
LSPRYQHTVTLYAKGLTCEADTFGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 366 bp

>AT178374 NZ_CP083990:651625-652041 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A0H2VCF5