Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3861644..3862256 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag IQS23_RS18505 Protein ID WP_000833473.1
Coordinates 3861644..3861829 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0A1AGA0
Locus tag IQS23_RS18510 Protein ID WP_022646255.1
Coordinates 3861846..3862256 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS18490 3857110..3858405 + 1296 WP_000985736.1 Fic family protein -
IQS23_RS18495 3858513..3860051 + 1539 WP_203038296.1 aldehyde dehydrogenase -
IQS23_RS18500 3860092..3861171 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
IQS23_RS18505 3861644..3861829 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
IQS23_RS18510 3861846..3862256 + 411 WP_022646255.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
IQS23_RS18515 3862368..3864347 - 1980 WP_022646254.1 glycoside hydrolase family 127 protein -
IQS23_RS18520 3864358..3865758 - 1401 WP_001600688.1 MFS transporter -
IQS23_RS18525 3865984..3866799 + 816 WP_119837365.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T178372 WP_000833473.1 NZ_CP063476:3861644-3861829 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T178372 NZ_CP083990:183644-183751 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15214.08 Da        Isoelectric Point: 4.4482

>AT178372 WP_022646255.1 NZ_CP063476:3861846-3862256 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALAAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT178372 NZ_CP083990:c183595-183529 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AGA0

References