Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3162460..3162957 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IQS23_RS15310 Protein ID WP_001173343.1
Coordinates 3162460..3162633 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IQS23_RS15315 Protein ID WP_000398619.1
Coordinates 3162661..3162957 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS15300 3159130..3160587 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
IQS23_RS15305 3160824..3162341 + 1518 WP_022646456.1 lysine--tRNA ligase -
IQS23_RS15310 3162460..3162633 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IQS23_RS15315 3162661..3162957 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IQS23_RS15320 3163186..3163458 - 273 WP_000405647.1 N-acetyltransferase -
IQS23_RS15325 3163470..3163700 - 231 WP_001683478.1 (4Fe-4S)-binding protein -
IQS23_RS15330 3163881..3165512 + 1632 WP_022646455.1 sensor histidine kinase -
IQS23_RS15335 3165509..3166228 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3153606..3162957 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T178370 WP_001173343.1 NZ_CP063476:c3162633-3162460 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T178370 NZ_CP083975:401105-401461 [Rhodococcus rhodochrous]
ATGATCCGCCGGGGTCATGTGCACTGGGTCGATCTTGATCCGGCGCAGGGGAGTGAAGCGGCCAAGCGTCGACCCGTCGT
TGTGGTGAGTAATGATCGCGCCAATGCGACAGCTGAGCGACTCGGCCGGGGGGTGGTCACCGTGGTGCCGTTGACGAATA
ACACGGCGCATGTGTTCGCGTTTCAGGTTCTCGTCGAACCTGACGAATCGGGCATGCCGCGTGAGTCGAAGGCTCAAGCG
GAGCAGGTTCGTTCGGTCTCGGTGCGTCGTCTCGACTCAGACCCGGTCGGGAAGCTCTCGACGAGAACGCTTGCCGCGCT
TGACGAAGCTCTGCGGTTGCATCTCGATCTGCGCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT178370 WP_000398619.1 NZ_CP063476:c3162957-3162661 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT178370 NZ_CP083975:400893-401108 [Rhodococcus rhodochrous]
ATGACGACGAGGTTCACCATCAGTTTGCCGGACGAAGACGCTCGCGTCCTCGACGCGTACGTTCGCGACCACGGTTTGGG
CGGCCGGTCGGCTGGGATTCGCGCCGCGCTTCGGATGCTCCGGGAGGTGGGACTCGAGCGCGATTATGCGACTGCTTTCG
TCGAAGGTGGTGCCGAGGATGACGACTGGGACGTCACTTCGGCTGATAGCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References