Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2802407..2802957 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag IQS23_RS13615 Protein ID WP_001160966.1
Coordinates 2802407..2802721 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag IQS23_RS13620 Protein ID WP_000125568.1
Coordinates 2802724..2802957 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS13590 2798082..2799368 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
IQS23_RS13595 2799368..2800357 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
IQS23_RS13600 2800354..2801175 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
IQS23_RS13605 2801178..2801555 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
IQS23_RS13610 2801562..2802410 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
IQS23_RS13615 2802407..2802721 - 315 WP_001160966.1 CcdB family protein Toxin
IQS23_RS13620 2802724..2802957 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
IQS23_RS13625 2803043..2803522 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
IQS23_RS13630 2803714..2805576 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
IQS23_RS13635 2805569..2806099 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
IQS23_RS13640 2806207..2807538 - 1332 WP_001183201.1 MFS transporter -
IQS23_RS13645 2807596..2807883 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T178368 WP_001160966.1 NZ_CP063476:c2802721-2802407 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T178368 NZ_CP083957:c46592-46059 [Mesorhizobium sp. B1-1-8]
GTGAAGGTCACCCGCGTCGGGCCGGACGAGATCTTCCATCGCTATCTGACACCCAAATGGGCCTTCCTGCCCACCAGCGG
TGCAGGCGCGGCGATCGACGGCGGGCGCTTCAACCGGCCAGGCGTCGAAGCGCTTTACCTGTCCCGCGCTCCTCAGACAG
CGTTGGAGGAATACAAGCAGGGTGCCAGCATCGTCCCGCCAGCCACTCTTGCCGCTTACAAGATCACGCTCGCCGAGGTG
GCCGACCTTTCGCAGGGATTCGACCCGGACATCTGGGACGGTGCCTGGCGGGAATGGGATTGTGCCTGGCGCAAGATCGC
TCGCATCGACAAGAAAATCCCGCCGTCGTGGAAACTTGCGGACATGGTCATCACCGCCGGGCTTCGCGGCATTCTGTTTC
CGTCGCTGCGTCATGCCGGCGGCACTAATCTGGTGATCTTTCCAGCCAACCTTGTCGACGGCGATCACGTCGCGGTTCAC
GATCCCGATCACCGACTACCGCACGACCAGTCGTCCTGGTCCTTGAGTCATTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT178368 WP_000125568.1 NZ_CP063476:c2802957-2802724 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT178368 NZ_CP083957:c46975-46589 [Mesorhizobium sp. B1-1-8]
ATGGCCCAGGCACAGCTAGTACATTCCACGGTTATCTCGGGCTTTGTCGACAGCCTGCAGGAGCCGCGCACTCCTTATAT
CTCGCCGAAGCGCCTGTCGCAGGCGCTGGGCGTGAAAGTTGCCAATCTGGCGCAATTGACCGGCGTCCACCGCAACACGC
TCCGCAACCCGTCATCGGAGCGCCTGCAGGGCCGGATGCGCGAGATGGTGAAGGTGATCTCGGCGGCAACGGAGCTCACC
GGCGACGTGGACAAGGCAATCTACTGGTATCGCAATGAACCGATCGCCGATTATGGCCATCGCACGGCGGCCGAGCTCGT
CGCGGACGGCCAGGTCGAAGCAGTCCTGGCCTTTATCCGGGATCTCGAGAACGGGGCGCGCGGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References