Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2386919..2387537 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IQS23_RS11675 Protein ID WP_001291435.1
Coordinates 2387319..2387537 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IQS23_RS11670 Protein ID WP_000344800.1
Coordinates 2386919..2387293 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS11660 2382008..2383201 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IQS23_RS11665 2383224..2386373 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IQS23_RS11670 2386919..2387293 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IQS23_RS11675 2387319..2387537 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IQS23_RS11680 2387709..2388260 + 552 WP_000102574.1 maltose O-acetyltransferase -
IQS23_RS11685 2388376..2388846 + 471 WP_022645280.1 YlaC family protein -
IQS23_RS11690 2389010..2390560 + 1551 WP_022645279.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IQS23_RS11695 2390602..2390955 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IQS23_RS11705 2391334..2391645 + 312 WP_000409911.1 MGMT family protein -
IQS23_RS11710 2391676..2392248 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T178366 WP_001291435.1 NZ_CP063476:2387319-2387537 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T178366 NZ_CP083956:4637357-4637650 [Mesorhizobium sp. B1-1-8]
TTGAAGATCGTCTGGTCAGCCCTCGCGCTTTCGGATCGTGATGCGATATTCACGCATATCGAAGCAGACAACCCCACGGC
CGCGATCGTTATCGACGAGCGGATTGTCGCCGCGACCAGCCGTCTTCGTAACTTCCCCGAGAGCGGTCGGCCTGGCAGAC
TTGCGGGAACCCGAGAGCTTGTCATCGTCGGGACGTCATACGTCGCAGCCTATCAGCTGAACGAGGACACTGTTCGAATC
CTGCGCGTGCTTCACGGAGCGCAGCGTTGGCCAGACGATCTGCCAGCCGGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT178366 WP_000344800.1 NZ_CP063476:2386919-2387293 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT178366 NZ_CP083956:4637052-4637360 [Mesorhizobium sp. B1-1-8]
ATGCCAGCGAACGCACTTATCCAGACACGCATCGACGCCGACATCCGGGATCGGGCTTCCGCGGTGCTCGAAAACATGGG
ACTCACGGTTTCGGATGCGGTGCGGATACTCCTCACCCGTACTGCCAACGAGGGTGCACTGCCTATCGATCTGCTCACAA
ACAGCGAGGCATATGACGTGTGGTTTCGCGCCAAGGTGCGCGAGGCCCTCGATGATATCCGGCCCGACATTCCCGACGAT
CTGGTCGAGGCGCATTTTGCCGTGCGACGCGCGGCAGCGCGTCGTAAGGCTGGTGAACCTAAACCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References