Detailed information of TA system TA178364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1944510..1945215
• Replicon: chromosome
• Accession: NZ_CP063476
• Organism: Escherichia coli strain EM10-18-28

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: IQS23_RS09500
• Protein ID: WP_000539521.1
• Coordinates: 1944510..1944896 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IQS23_RS09505
• Protein ID: WP_001280945.1
• Coordinates: 1944886..1945215 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS23_RS09480	1940514..1941140	+	627	WP_001295292.1	glutathione S-transferase GstB	-
IQS23_RS09485	1941137..1942252	-	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
IQS23_RS09490	1942363..1942746	-	384	WP_000497137.1	biofilm formation regulator BssR	-
IQS23_RS09495	1942959..1944284	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
IQS23_RS09500	1944510..1944896	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IQS23_RS09505	1944886..1945215	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
IQS23_RS09510	1945285..1946613	-	1329	WP_022645414.1	GGDEF domain-containing protein	-
IQS23_RS09515	1946621..1948968	-	2348	Protein_1872	EAL domain-containing protein	-
IQS23_RS09520	1949145..1950056	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T178364 WP_000539521.1 NZ_CP063476:1944510-1944896 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T178364 NZ_CP083956:c4065158-4064901 [Mesorhizobium sp. B1-1-8]
ATGAAGATCGTTTGGGATGAGCCGAAGCGGCTGGCCAACATCGATAAACACGGCTTGGATTTCGCGGCGTTGGATGAAGA
GTTCTTCCTTGCGTCGACGATTCGGGCGGCCAAGGCCGGTCGCTTCATGGCGATTGGTCGGGTAGCTGGCGGCGTTGTTG
CGGTGGTCTTTGCCCGGCTTGGCTCGGAAGGCTTTTCGATGATTTCGATGCGTCCGGCCAACCAGGCGGAAAGGAGATTG
TTCGATGGCAAAACCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT178364 WP_001280945.1 NZ_CP063476:1944886-1945215 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT178364 NZ_CP083956:c4064914-4064588 [Mesorhizobium sp. B1-1-8]
ATGGCAAAACCTAAGCTGAACGTTACGACACCTGACAAGGAGTTCCAGCCAGGCAGAGGCTTCACGAAGGAAGATTGGGA
CGCCGTTTCCGACAATCCCGAGTGGACCGAGGAGGATTTTCGAAACGCCCGTCCCTTCACCGAGGTTTTTCCCGATCTTG
CCGAAAGCATTCGCCGCTCGCGCGGCCGGCCGGCGCTCGACAATCCGAAGAAGCAGGTGACACTTCGTCTCGACAGCGAC
GTGGTCGCCCGCTTTCGCGCCGGCGGGCCAGGTTGGCAGAGCCGGATCAACGATATCCTGCGCAAGGCTGCCGGTCTTCA
AAAATAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6