Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1689900..1690684 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag IQS23_RS08305 Protein ID WP_000613626.1
Coordinates 1690190..1690684 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag IQS23_RS08300 Protein ID WP_024946541.1
Coordinates 1689900..1690193 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS08290 1685061..1686020 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
IQS23_RS08295 1686593..1689766 + 3174 WP_063814920.1 ribonuclease E -
IQS23_RS08300 1689900..1690193 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
IQS23_RS08305 1690190..1690684 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
IQS23_RS08310 1690779..1691732 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
IQS23_RS08315 1691744..1693387 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
IQS23_RS08320 1693453..1694394 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
IQS23_RS08325 1694394..1695491 - 1098 WP_021580666.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T178363 WP_000613626.1 NZ_CP063476:1690190-1690684 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T178363 NZ_CP083956:c3959992-3959717 [Mesorhizobium sp. B1-1-8]
ATGGAGTTGAATTGGACCAGAAAGGCATTGTCCGATCTGGTCAGGCTTCATGATTTTCTTGCTCCGGTGAATAGTCAGGC
CGCTGCTCGTGTGGTGCAGTCACTCGCCGCCGCGCCGAAACGGCTAGTGGAATATCCACGCATCGGTGAGAGACTTGAAC
AGTACGAGCCACGCGAGGTGCGTCGCATCCTGGTAGGCAATTACGAAATTCGGTACGAAATCCGGAACAGCACAATTTTG
GTGCTGCGTCTGTGGCATACCCGAGAGGATCGATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT178363 WP_024946541.1 NZ_CP063476:1689900-1690193 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT178363 NZ_CP083956:c3960252-3959992 [Mesorhizobium sp. B1-1-8]
ATGGATACTAAGGTCATCACCGCTCACGTTCCTTTGCCACTTGCTGAGAAAGTGGACCAACTTGCCGCGCGTCTGGAGCG
CCCGCGCGGCTGGATTGTCAAACAGGCTCTTTCCGCTTGGGTCGATCTGGAGGAGGAGCGTCACAAGCTTACGCTTGAAG
CACTGGCTGAGGTGGATGCTGGCAGGATCATAGACCACCAAGCGGTCCAGGCCTGGGCCGACAGCCTTGGGACTGAAAAG
CCGGTGCCGGTGCCGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References