Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1283813..1284451 Replicon chromosome
Accession NZ_CP063476
Organism Escherichia coli strain EM10-18-28

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag IQS23_RS06280 Protein ID WP_000813794.1
Coordinates 1284275..1284451 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag IQS23_RS06275 Protein ID WP_001270286.1
Coordinates 1283813..1284229 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS23_RS06255 1278965..1279906 - 942 WP_022645667.1 ABC transporter permease -
IQS23_RS06260 1279907..1280920 - 1014 WP_022645666.1 ABC transporter ATP-binding protein -
IQS23_RS06265 1280938..1282083 - 1146 WP_000047466.1 ABC transporter substrate-binding protein -
IQS23_RS06270 1282328..1283734 - 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
IQS23_RS06275 1283813..1284229 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
IQS23_RS06280 1284275..1284451 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
IQS23_RS06285 1284673..1284903 + 231 WP_000491567.1 DUF2554 family protein -
IQS23_RS06290 1284995..1286956 - 1962 WP_203038301.1 U32 family peptidase -
IQS23_RS06295 1287029..1287565 - 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
IQS23_RS06300 1287618..1288829 + 1212 WP_071788120.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T178362 WP_000813794.1 NZ_CP063476:c1284451-1284275 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T178362 NZ_CP083956:3918003-3918197 [Mesorhizobium sp. B1-1-8]
ATGCTAGAGCGTGACAGTCGAAAGATCGTGAAGCGCTTGAAGGATGACGGCTTCGAGCTGGTTTCGGTGCGCGGTTCACA
TCACAAGTTCCGCAAAGGCGAAATCACATTGATCGTCCCGCATCCGGAAAAGGATCTGCCGACCGGCACAGCTCGTGCGA
TCGCCAAGCAAGCAGGCTGGATAAGGGCAGGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT178362 WP_001270286.1 NZ_CP063476:c1284229-1283813 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT178362 NZ_CP083956:3918216-3918614 [Mesorhizobium sp. B1-1-8]
ATGAAGAATTATTTTGCATTGGTCGACAAGGACGCCGATAGCGCGTTCGGCATCCGCTTTCCCGATATTCCCGGCTGTTT
TTCGGCTGCCGACGACGCGAAGGACATCGTGCCGAACGCGACCGAGGCACTGCAACTGTGGGCAGAGGACATGCCTCTTC
CCGAACCGTCCAGCCACGAAGCGGTTATTGCGCTTCCCGACATACGCCGTGCGCTGGCTGACGGCAGCTATCTGGTCTCC
GTGCCACTCATCGACAATGACAGCGCCGTCGTCCGGGCGAACGTGACGTTCGAGCGCGGCGTTCTGCGTGCGATCGATGC
CGCCGCGCGCGAACGCGGCATTACCAGATCGGCGTTCCTTTCCAGCGCGGCCCGCAAGGAGATCGAAGCAAAGCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References