Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 1436..1958 Replicon plasmid pEM18-18-42_2
Accession NZ_CP063475
Organism Escherichia coli strain EM18-18-42

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IQS24_RS23875 Protein ID WP_000220560.1
Coordinates 1436..1717 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IQS24_RS23880 Protein ID WP_000121743.1
Coordinates 1707..1958 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS24_RS23855 50..379 - 330 WP_000866648.1 hypothetical protein -
IQS24_RS23860 472..687 - 216 WP_001180116.1 hypothetical protein -
IQS24_RS23865 677..922 - 246 WP_000356546.1 hypothetical protein -
IQS24_RS23870 967..1290 - 324 WP_001181903.1 hypothetical protein -
IQS24_RS23875 1436..1717 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IQS24_RS23880 1707..1958 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IQS24_RS23885 3214..4050 + 837 WP_001050931.1 replication initiation protein -
IQS24_RS23890 4090..4536 + 447 WP_001074386.1 hypothetical protein -
IQS24_RS23895 4604..5026 + 423 WP_031942371.1 DNA distortion polypeptide 3 -
IQS24_RS23900 5086..6054 - 969 WP_166501310.1 IS5 family transposase -
IQS24_RS23905 6056..6349 - 294 WP_015060510.1 hypothetical protein -
IQS24_RS23910 6365..6595 - 231 WP_000051064.1 partitioning protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / tet(A) / qnrS1 / blaTEM-1B - 1..47868 47868


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T178351 WP_000220560.1 NZ_CP063475:c1717-1436 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T178351 NZ_CP083952:3229557-3229982 [Mesorhizobium sp. B2-1-8]
ATGATCCTCATCGACACCAATGTCATTTCCGAGCCGTGGAGACCTGCACCCGATCCCCGCGTCATCGGGTGGATCGACGC
CCAGGCGGTCGAAACCCTGTATCTCTCGGCTGTCACCGTAGCCGAGTTGCGTTTCGGCATCGCGGCGATGCCTGCCGGCA
AGCGCCGCACGATCCTGCAGGATCGTCTCGAGCAGGACGCGTTGCCGCTGTTTGCTGGCCGCATTCTGCCCTTCGACCTC
GATGCGTCGCGCGCCTATGCCAACCTCATGGTACAGGCGAGAGCCGCGGGCAAGGCGATCGGCAACGCGGACGGCTACAT
CGCGGCCACTGCCGTAGCGCACGGTCTCACGATCGCCACACGCGACACCGGTCCATTCGCGGCCGCCGGGTTGAACACCG
TCAATCCGTGGGAAGCAGCGATATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT178351 WP_000121743.1 NZ_CP063475:c1958-1707 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT178351 NZ_CP083952:3229306-3229560 [Mesorhizobium sp. B2-1-8]
ATGAGTGCTGTCACTATCCGCAATTTGCCAGAGGAAACGCTCCGCGCCCTGAAAGTGCGGGCAGCGCATCACGGCCGCAG
CACCGAAGCCGAAATGCGTGAAATCCTGGAAACGGCCGTGCGGCCACCCAGTCGGGTCAAACTCGGCTCCCTGCTCGCCG
CCATCGGCCGCGAGGCCGGATTGTCGGATGGGGACGTCGATGCCTTGCAGCAGCTCCGCGACAAGACACCGGGCGGGCCG
GTGAGATTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References