Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3664950..3665604 Replicon chromosome
Accession NZ_CP063473
Organism Escherichia coli strain EM18-18-42

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IQS24_RS17660 Protein ID WP_000244777.1
Coordinates 3664950..3665357 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS24_RS17665 Protein ID WP_000354046.1
Coordinates 3665338..3665604 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS24_RS17640 3660907..3662640 - 1734 WP_205595474.1 single-stranded-DNA-specific exonuclease RecJ -
IQS24_RS17645 3662646..3663356 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS24_RS17650 3663381..3664277 - 897 WP_205595475.1 site-specific tyrosine recombinase XerD -
IQS24_RS17655 3664389..3664910 + 522 WP_001055874.1 flavodoxin FldB -
IQS24_RS17660 3664950..3665357 - 408 WP_000244777.1 toxin CptA Toxin
IQS24_RS17665 3665338..3665604 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS24_RS17670 3665847..3666827 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IQS24_RS17675 3667023..3667682 - 660 WP_000250274.1 hemolysin III family protein -
IQS24_RS17680 3667846..3668157 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IQS24_RS17685 3668202..3669635 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
IQS24_RS17690 3669692..3670435 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T178348 WP_000244777.1 NZ_CP063473:c3665357-3664950 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178348 NZ_CP083952:c2718839-2718651 [Mesorhizobium sp. B2-1-8]
ATGACTAGCGCTGAAATCATCCGTCTGTTGGAAGCTGACGGTTGGTTCGAGGTCGCCCGAAAGGGTAGCCATGCGCAATT
CAAACACCGCGACAAGCCAGGGCGCGTCACCGTTCCCCATCCAAAGCGCGATATTCCGATAGGCACTTTGCGCAGCATCG
AAAAGCAATCTGGACTGAAACTGAGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178348 WP_000354046.1 NZ_CP063473:c3665604-3665338 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178348 NZ_CP083952:c2718645-2718244 [Mesorhizobium sp. B2-1-8]
ATGAAGCAGTATATCGGACTTATCCACAAGGATGCCGACAGCGACTTCGGCGTGTCTTTCCCTGATTTCCCAGGCGTGAT
TACCGCCGGCAAGGATCTGGATGACGCCCGGTCCATGGCTGAAGAGGCGCTTGCCCTCCATATCGAAGGGCTGGTCGAGG
ATGGCGATGCCATTCCGGAGCCTTCCAGTCTGGAGACAGTGATGACAGACGCCGACAATCGTGACGGCGTTGCAATCCTG
GTCGCGGCAAGAACCGGGGCCAGGAAGGCGGTGCGTATCAACGTGACGCTGCCGGACGATGTTCTCCAGCGGATCGATGC
CTTTGCCGAAGCCCACGGCTATACGCGATCGGGGTTTCTGGCGAAGGCGGCGGAGAAGGTGATGGCGCTTGAAGCGGCCT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References