Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3539194..3539777 Replicon chromosome
Accession NZ_CP063473
Organism Escherichia coli strain EM18-18-42

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag IQS24_RS17140 Protein ID WP_000254738.1
Coordinates 3539194..3539529 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag IQS24_RS17145 Protein ID WP_000581937.1
Coordinates 3539529..3539777 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS24_RS17125 3535081..3536379 - 1299 WP_000036723.1 phosphopyruvate hydratase -
IQS24_RS17130 3536467..3538104 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IQS24_RS17135 3538332..3539123 - 792 WP_001400925.1 nucleoside triphosphate pyrophosphohydrolase -
IQS24_RS17140 3539194..3539529 - 336 WP_000254738.1 endoribonuclease MazF Toxin
IQS24_RS17145 3539529..3539777 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IQS24_RS17150 3539855..3542089 - 2235 WP_000226815.1 GTP pyrophosphokinase -
IQS24_RS17155 3542137..3543438 - 1302 WP_160528344.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T178347 WP_000254738.1 NZ_CP063473:c3539529-3539194 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T178347 NZ_CP083952:c2321553-2321272 [Mesorhizobium sp. B2-1-8]
ATGATACGTTCATTCAAGGACAAGACCAGCGAAGCCGTTTCGAACGGCAAGGCTCCAAAGGGCTTCCCTTCGGATCTTGT
CCGACCGGCCCAATGGAAGCTGTACATGATCGACAACGCCGCCGAACTGTCAGATCTCAAGACGCCTCCCGGCAACAGGC
TTGAGCCGTTGAAAGGCAACCGTGCCGGTCAGCATTCGATCCGCATCAACGATCAGTTTCGCATCTGCTTTCGCTGGGTG
GATGGGCAAGCAGAAGATGTTGAAATAACCGACTACCATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT178347 WP_000581937.1 NZ_CP063473:c3539777-3539529 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT178347 NZ_CP083952:c2321257-2320958 [Mesorhizobium sp. B2-1-8]
ATGCTTGATATCAACCCGCCTATCCATCCCGGAGAAATTCTCCGCGAAGAGTATCTCATACCGCTTGGTCTGAAGCCTTA
CACGCTGGCCAAAAAGCTGCATGTGCCGCGCACGCGCATCGAGCGCCTGGCCAGCGAGACGACCCCGGTAACCCCTGATA
CCGCGTTGCGCCTTGCGAAGTTTTTTGGGACCACGCCGCAGTTCTGGATGAACATGCAGGCAAGCTATGACCTCGCCGTC
GAGGGAAAGGCCAAGAGAGCCGACCTTGAAACGATCGAACGATTGGAGACTGCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References