Detailed information of TA system TA178335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1545133..1545838
• Replicon: chromosome
• Accession: NZ_CP063473
• Organism: Escherichia coli strain EM18-18-42

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: IQS24_RS07495
• Protein ID: WP_000539521.1
• Coordinates: 1545452..1545838 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IQS24_RS07490
• Protein ID: WP_001280945.1
• Coordinates: 1545133..1545462 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS24_RS07470	1541098..1541316	+	219	WP_205595638.1	hypothetical protein	-
IQS24_RS07475	1541327..1542023	-	697	Protein_1444	IS1 family transposase	-
IQS24_RS07480	1542078..1543727	+	1650	Protein_1445	EAL domain-containing protein	-
IQS24_RS07485	1543735..1545063	+	1329	WP_000086877.1	GGDEF domain-containing protein	-
IQS24_RS07490	1545133..1545462	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
IQS24_RS07495	1545452..1545838	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IQS24_RS07500	1546064..1547389	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
IQS24_RS07505	1547602..1547985	+	384	WP_000497137.1	biofilm formation regulator BssR	-
IQS24_RS07510	1548096..1549211	+	1116	WP_000554946.1	aldose sugar dehydrogenase YliI	-
IQS24_RS07515	1549208..1549834	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1541327..1541620	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T178335 WP_000539521.1 NZ_CP063473:c1545838-1545452 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T178335 NZ_CP083950:1333375-1333650 [Mesorhizobium sp. B4-1-4]
ATGGAGTTGAAATGGACCAGTAAGGCTTTGTCCGATCTGGTCCGGCTCCATGACTTTCTAGCGCCGGCAAATGGTCCGGC
TGCCGCACGAGCCGTACAGTCGCTTGCCGCCGCGCCGGCACGGCTGGTTGAGTATCCACGCATAGGTGAGAGACTGGAAG
AGTTCGAACCACGCGAGGTGCGCCGCATTTTGGTAGGCAGCTACGAAATTCGTTACGAAATACAGAGCACGACAATCTTC
GTGTTGCGCCTATGGCATACGCGGGAGGATCGATAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT178335 WP_001280945.1 NZ_CP063473:c1545462-1545133 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT178335 NZ_CP083950:1333115-1333375 [Mesorhizobium sp. B4-1-4]
ATGGACACAAAAGTAATCACCGCTCACGTTCCGCTGCCGCTGGCGGAAAAAGTAGACCAACTGGCTGCTCGCCTTGAGCG
CCCGCGCGGCTGGATTGTCAAACAAGCTCTTTCTGCGTGGGTGGATTTGGAGGAGGAGCGCCAACGGCTCACGCTGGAAG
CACTGGCCGATGTGGACGCAGGACGGGTCATCGATCACCAGGCCGTGCAGGCATGGGCTGAGAGTCTTGGCACCGAAAAG
CCGCTGCCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6