Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1083884..1084502 Replicon chromosome
Accession NZ_CP063473
Organism Escherichia coli strain EM18-18-42

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IQS24_RS05135 Protein ID WP_001291435.1
Coordinates 1083884..1084102 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IQS24_RS05140 Protein ID WP_000344800.1
Coordinates 1084128..1084502 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS24_RS05095 1078999..1079310 - 312 WP_000409911.1 MGMT family protein -
IQS24_RS05105 1079689..1079775 + 87 Protein_986 DUF1428 family protein -
IQS24_RS05110 1079827..1080524 + 698 Protein_987 IS1 family transposase -
IQS24_RS05115 1080541..1080819 + 279 Protein_988 DUF1428 family protein -
IQS24_RS05120 1080861..1082411 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IQS24_RS05125 1082575..1083045 - 471 WP_000136192.1 YlaC family protein -
IQS24_RS05130 1083161..1083712 - 552 WP_000102564.1 maltose O-acetyltransferase -
IQS24_RS05135 1083884..1084102 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IQS24_RS05140 1084128..1084502 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IQS24_RS05145 1085048..1088197 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IQS24_RS05150 1088220..1089413 - 1194 WP_205595608.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1080231..1080524 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T178334 WP_001291435.1 NZ_CP063473:c1084102-1083884 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T178334 NZ_CP083949:588-869 [Acinetobacter johnsonii]
ATGGCTATCAAACTTCGTAAATGGGACAGTGCTGAACATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATAGATGAATCTAATGGTGATGCGGCATTCATTGCAAAAGCTTTAGGTAATATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCAAGAGACACAGGTTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGCGATGTAAATCCAAGCTTTGATACTGTT
ATTAAAGTGGTAAAAGCGCTGAATTTACGCTTAGCAATTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT178334 WP_000344800.1 NZ_CP063473:c1084502-1084128 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT178334 NZ_CP083949:285-587 [Acinetobacter johnsonii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTTACTAAGTTAAAAGACCAGCAGGCAAAAAGACGTATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTCTCTGAATTACGTTTCT
TCTTTGGACCTGGCTACAGGATCTATTACTGTAAGCAAGGGCAAAGGGTCGTTATTCTTTTAGCAGGCGGAGACAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAACTAGCACAAGATTTAGAGGAGGAGCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References