Detailed information of TA system TA178333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1048794..1049631
• Replicon: chromosome
• Accession: NZ_CP063473
• Organism: Escherichia coli strain EM18-18-42

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: IQS24_RS04955
• Protein ID: WP_000227784.1
• Coordinates: 1048794..1049336 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: IQS24_RS04960
• Protein ID: WP_001297137.1
• Coordinates: 1049320..1049631 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS24_RS04925	1043923..1044834	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
IQS24_RS04930	1045002..1045493	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
IQS24_RS04935	1045621..1046985	-	1365	WP_001000978.1	MFS transporter	-
IQS24_RS04940	1047333..1047458	+	126	Protein_954	sel1 repeat family protein	-
IQS24_RS04945	1047514..1048211	+	698	Protein_955	IS1 family transposase	-
IQS24_RS04950	1048208..1048738	+	531	WP_205595698.1	hypothetical protein	-
IQS24_RS04955	1048794..1049336	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
IQS24_RS04960	1049320..1049631	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
IQS24_RS04965	1049816..1050706	-	891	WP_000971336.1	heme o synthase	-
IQS24_RS04970	1050718..1051047	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
IQS24_RS04975	1051047..1051661	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
IQS24_RS04980	1051651..1053642	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
IQS24_RS04985	1053664..1054611	-	948	WP_001239440.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1047918..1048211	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T178333 WP_000227784.1 NZ_CP063473:c1049336-1048794 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T178333 NZ_CP083949:285-587 [Acinetobacter johnsonii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTTACTAAGTTAAAAGACCAGCAGGCAAAAAGACGTATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTCTCTGAATTACGTTTCT
TCTTTGGACCTGGCTACAGGATCTATTACTGTAAGCAAGGGCAAAGGGTCGTTATTCTTTTAGCAGGCGGAGACAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAACTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT178333 WP_001297137.1 NZ_CP063473:c1049631-1049320 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT178333 NZ_CP083949:588-869 [Acinetobacter johnsonii]
ATGGCTATCAAACTTCGTAAATGGGACAGTGCTGAACATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATAGATGAATCTAATGGTGATGCGGCATTCATTGCAAAAGCTTTAGGTAATATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCAAGAGACACAGGTTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGCGATGTAAATCCAAGCTTTGATACTGTT
ATTAAAGTGGTAAAAGCGCTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3