Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 306689..307186 Replicon chromosome
Accession NZ_CP063473
Organism Escherichia coli strain EM18-18-42

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag IQS24_RS01395 Protein ID WP_160528392.1
Coordinates 307013..307186 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IQS24_RS01390 Protein ID WP_000398619.1
Coordinates 306689..306985 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS24_RS01370 303420..304139 - 720 WP_000611288.1 two-component system response regulator DcuR -
IQS24_RS01375 304136..305767 - 1632 WP_001216477.1 sensor histidine kinase -
IQS24_RS01380 305948..306178 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
IQS24_RS01385 306190..306462 + 273 WP_000405651.1 N-acetyltransferase -
IQS24_RS01390 306689..306985 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IQS24_RS01395 307013..307186 + 174 WP_160528392.1 type V toxin-antitoxin system toxin GhoT Toxin
IQS24_RS01400 307305..308822 - 1518 WP_001295074.1 lysine--tRNA ligase -
IQS24_RS01405 309059..310516 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6569.09 Da        Isoelectric Point: 10.2288

>T178329 WP_160528392.1 NZ_CP063473:307013-307186 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLIGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T178329 NZ_CP083946:c2609-2322 [Aeromonas hydrophila]
ATGACCTTTGAGCTTGAGTTTGATCCCCGTGCTTGGCGGGAGTGGAAGAAACTGGGCGATACCGTGCGCCAGCAGTTCAA
GAAGAAGCTGGTAGAGGTGCTGGAACGGCCAAGAAATGAGGCCAACCGCCTGCATGGGCTGCCGGATTGCTACAAGGTCA
AATTGCGTGGTGCCGGATACCGGTTGGTGTACCAGGTGCAAGATAATCGGGTGGTGGTGTTTGTGGTCGCGGTAGGGAAA
CGAGAGCGTGAGCAGGTATATCTGGACGCAGGTTCGCGGCTCGAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT178329 WP_000398619.1 NZ_CP063473:306689-306985 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT178329 NZ_CP083946:c2841-2599 [Aeromonas hydrophila]
ATGGGAACGATCAATTTGCGCATCGATGATGATCTAAAAGCCCGTTCTTATGCGGTGCTGGATCGGTTGGGGGTGACGCC
TTCAGAGTTGCTGCGGCAAACGCTGGAGTACGTTGCTGCTCAGGAGCGGTTACCGTTTCGGACTACCGTCGTGACAGAGG
ACGAAGAGGCGCTTCTTGCGCTCGTTCGGGAGCGGTTGGCCAGCCCCCAAAAAGGGATCAAGGTGTCGCTGGATGACCTT
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References