Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4177174..4177828 Replicon chromosome
Accession NZ_CP063467
Organism Escherichia coli strain EM20-18-50

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IQS25_RS20075 Protein ID WP_000244777.1
Coordinates 4177421..4177828 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IQS25_RS20070 Protein ID WP_000354046.1
Coordinates 4177174..4177440 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS25_RS20045 4172343..4173086 + 744 WP_000951964.1 SDR family oxidoreductase -
IQS25_RS20050 4173143..4174576 - 1434 WP_157924568.1 6-phospho-beta-glucosidase BglA -
IQS25_RS20055 4174621..4174932 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IQS25_RS20060 4175096..4175755 + 660 WP_000250274.1 hemolysin III family protein -
IQS25_RS20065 4175951..4176931 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IQS25_RS20070 4177174..4177440 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IQS25_RS20075 4177421..4177828 + 408 WP_000244777.1 toxin CptA Toxin
IQS25_RS20080 4177868..4178389 - 522 WP_001055874.1 flavodoxin FldB -
IQS25_RS20085 4178501..4179397 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IQS25_RS20090 4179422..4180132 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IQS25_RS20095 4180138..4181871 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T178326 WP_000244777.1 NZ_CP063467:4177421-4177828 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178326 NZ_CP083938:c5221046-5220168 [Comamonas thiooxydans]
ATGCACGAGGATTTGCTGTCGCTGAAACTGCAGCTGCTGCAGACCGGTTTTCCCGAGCGGCTGGTGAAGCTTCCCCAGGG
CGCCCAGGTCGCCTGGCGTGAGGCAGGCGACGCCAGCTCCGACTTTGCGGTGGTGCTGCTGCATGGCATCAGCTCCGGGG
CTGCATCCTGGCTGGATGTGGCGCTGCAGCTGGCTGACAAAGTGCGTGTCCTGGCCTGGGATGCACCCGGTTATGGCGTG
TCGACGCCGCTGGCACAGTCGGCGCCTGCCGATGCCGACTATGCGCAGGCGCTGGCCCAGTCGCTGCTGGTGCTGGGCGT
GCGGCGCTGCATCCTGGTCGGGCACTCGCTGGGCGCCCTGATGGCAGCCAGGCTGGCTGCCATGGCAGCACCAGGACTGG
TCGAGCAACTGGTGCTCATCAGCCCGGCGGGCGGCTACGGCGCTGCGGCCAAGGCCGAGCAGCAGTCCAAGGTGCGCGAG
GGCCGGCTGGCGGCTCTGGCCGAGAAGGGCGTCGCCGGTCTGGCGGCCGTCATCGACCAGCGTCTGGTGTCCTCCGAGGC
TCCTGAAGTCGTGCGTGCATGGGTGCGCTGGAATACGGCGCGCATGCAGCCGCAGGGCTATGCGCAGGCGGTCGAGCTGC
TTTGCGGCAGCGATCTGGCACAGGCGCAGGGCAGGCTTGGCATGCCGGTCGAGGTTTGGGTCGGCGAGCACGATGTGGTG
ACTACACCTGCAGCTTGCAAGTCCTGGTCCGAAATGCTGGGCGCGCACTACGGTACGCTTGCGGCTGCCGGTCATGCTTC
ACCCGTGGAACAACCAATGGAGGTCGCGCATAGGCTGGCATCTTTGTTGAACCAAAGCTATTGCCCCAATACCAGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178326 WP_000354046.1 NZ_CP063467:4177174-4177440 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178326 NZ_CP083938:c5220162-5219356 [Comamonas thiooxydans]
ATGAGCGAAACCTCCGAGATTTCAACCAACGAACAAGACCGTTACACCGTGCCCGCTCTGGACCGAGGACTGCGCCTGCT
GGCCTGCTTTGGCCCGGCGCAACCGGTCTGGAGTGCGCCCGAGCTGGCGCGCAGCCTCGAGCTGCCGCGCTCCACGGTGT
TTCGCATGTTGACCACGCTTGAAAACTCGGGGTATCTGCAGCGCAGCGGCACCGAATACCGGCTCGGTCTGGCCGTGTTG
CGCCTGGGCTATGACTACCTGTCCACCCAGCCGCTGGCACAACTGGCCGAGCCTGTGCTGCAGGCCTTGTGCGATGAGCT
GGGCATGACCAGCAATCTTGCATTGCGCGACGGCACTTCCGTGGTCTATGTGGCTCGCGTCACTCCGTCCGGAGCATTCC
AGGGCGCTGTGCGTGTGGGATCGCGTCTGCCGGCGCATGCAACGGTGCTTGGACGGGCCCTGTTGCATGACATGGACGGC
GCGCAGCTGCGCTCGGTGTTTGGCGGCGAGGAACTGCCCCAGTTCTCCGAGAGCACGCCGCGCAATGTCGACGAGCTGCT
GCGCCTGCTGGCCGAGGATCGCGAGCGTGGTTATGCCATGGGAGAGGGCTTTTACGAGCCTGGCGTTTCCAGTATTGCAG
CGCCGGTGCGCGCCGCAGATGGGCGGGTGGTTGCGGGGCTGGCCATGGCCATTCCGTTTACCGAGCTGGGGCCCGAAAAA
ACCCAGCTCTGGGTGCAGAAGGTGCAGCAGGCGGCAGAGACTCTGTCCGCTCATTTGCGCCAGCAGCACCCGGACCATTT
GCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References