Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2903146..2903643 Replicon chromosome
Accession NZ_CP063467
Organism Escherichia coli strain EM20-18-50

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IQS25_RS14025 Protein ID WP_001173343.1
Coordinates 2903146..2903319 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IQS25_RS14030 Protein ID WP_000398619.1
Coordinates 2903347..2903643 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS25_RS14015 2899816..2901273 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
IQS25_RS14020 2901510..2903027 + 1518 WP_001295074.1 lysine--tRNA ligase -
IQS25_RS14025 2903146..2903319 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IQS25_RS14030 2903347..2903643 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IQS25_RS14035 2903872..2904144 - 273 WP_000405651.1 N-acetyltransferase -
IQS25_RS14040 2904156..2904386 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
IQS25_RS14045 2904567..2906198 + 1632 WP_112935020.1 sensor histidine kinase -
IQS25_RS14050 2906195..2906914 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T178323 WP_001173343.1 NZ_CP063467:c2903319-2903146 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T178323 NZ_CP083938:c1933270-1933088 [Comamonas thiooxydans]
GTGGTCGTAAAAATCAGCGAATTCAAGCGGTGGCTTGAAGCGCAGGAGGTCAGAGTCGAGAACGGTACGAATCACTGGAG
GCTGTACCACAACGGCAATGTAAGCACCCTGCCAAGACACCCAAGTAAAGAGCTGAAGGAAGGCACAAGACGCGCAATCC
TGAAGCAACTAGGAATCACTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT178323 WP_000398619.1 NZ_CP063467:c2903643-2903347 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT178323 NZ_CP083938:c1933022-1932594 [Comamonas thiooxydans]
ATGCAAAAGTACCCTGCCCGCTTCGATCCCGCCGACGAAGGGGGCTACACCGTCAGCTTCCGGGATATTCCGGAAGCCCT
CACCCAGGGAGATGACCTCGCGGATGCCGAGTCCATGGCCCGGGACGCGCTGGTCACGGCCATGGACTTCTACTTTGAAG
ATGGCCGCGCCGTACCTGCCCCCAGCAAGCCCGAAAAAGGCGAGCGCCTGGTGGAGTTGCCCGCCAGTGTCGCCGTCAAG
GTGGCCCTGCTCAATGCCCGGTTGGCCACGGGCGCCCGCCCCGCCGATGTGGCCCGCGCTATGGGTGTGAAACCGCAGGA
AATGACCCGCGTTTTTGACCTGGGCCATGCCACCAAAATCGACACAGTGGCAGCCGCACTGGCTGCAATGGGGTATGAGC
TGGAGTTGAACGTCCGGCGGGTGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References