178307

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 74825..75372 Replicon plasmid pEM22-18-53_5
Accession NZ_CP063464
Organism Escherichia coli strain EM22-18-53

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag IQS26_RS24495 Protein ID WP_000969996.1
Coordinates 74825..75106 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag IQS26_RS24500 Protein ID WP_000079928.1
Coordinates 75103..75372 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS26_RS24460 69836..70078 + 243 WP_000844627.1 transposase -
IQS26_RS24465 70110..70760 - 651 WP_000164043.1 tetracycline resistance transcriptional repressor TetR(A) -
IQS26_RS24470 70866..72065 + 1200 WP_000804064.1 tetracycline efflux MFS transporter Tet(A) -
IQS26_RS24475 72097..72957 - 861 WP_000493383.1 EamA family transporter -
IQS26_RS24480 72966..73580 - 615 Protein_85 helix-turn-helix domain-containing protein -
IQS26_RS24485 73542..73700 + 159 WP_197711125.1 MerR family transcriptional regulator -
IQS26_RS24490 73937..74785 - 849 WP_001057996.1 3'-5' exonuclease -
IQS26_RS24495 74825..75106 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IQS26_RS24500 75103..75372 - 270 WP_000079928.1 hypothetical protein Antitoxin
IQS26_RS24505 75427..75585 - 159 WP_000841008.1 hypothetical protein -
IQS26_RS24510 76281..77312 - 1032 WP_000907875.1 replication initiation protein -
IQS26_RS24515 78285..78548 + 264 WP_001324596.1 ash family protein -
IQS26_RS24520 78466..78753 + 288 WP_000074849.1 conjugal transfer protein TraA -
IQS26_RS24525 79195..79728 + 534 WP_001303319.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / qacE / sul1 / tet(A) - 1..94799 94799


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T178307 WP_000969996.1 NZ_CP063464:c75106-74825 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T178307 NZ_CP083931:1584408-1584674 [Alysiella filiformis DSM 16848]
ATGTTTGAATTTGAATACGACCCAAACAAAAGCCAAAGCAATCACACCAAACACGGCATTGATTTTGAAACCGCACAATA
TTTGTGGCAAGACCCCCAATTATTGAGCATTCCCATTCAAACAGAGCCTGAAATCCGTCATCTTGCCATTGGCATCATTG
GCGAAAAACATTGGTCAGCCATTTACACGCATCGCAATGGCAAAATTCGTTTAATTTCAGTTCGCCGTTCACGTGATAAG
GAGAAAATGCTGTATGAAACCCATTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT178307 WP_000079928.1 NZ_CP063464:c75372-75103 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT178307 NZ_CP083931:1584661-1584915 [Alysiella filiformis DSM 16848]
ATGAAACCCATTGATACCGAAGTTTTTGAGCGAAAGTTTGATGACAATCAAGAAGATATTTTAGAATATTTGGATTTGAG
TCAAGCCACACGCGTTCATCAAAACAAAATCGCCACACGCCGCGTAAACGTGGATTTTCCATTGTGGATGCTGGAAAGTT
TAGACCGTGAAGCGGCAAATTTGGGGGTTGCACGTCAAGCCTTAATTAAAATGTGGCTGTCGCAACACCTGCCACCTAAT
CAACAAAATATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References