178296

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 84409..85052 Replicon plasmid pEM22-18-53_3
Accession NZ_CP063462
Organism Escherichia coli strain EM22-18-53

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag IQS26_RS23540 Protein ID WP_001034046.1
Coordinates 84409..84825 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag IQS26_RS23545 Protein ID WP_001261278.1
Coordinates 84822..85052 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS26_RS23525 79546..79962 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
IQS26_RS23530 79959..80189 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
IQS26_RS23535 80570..84364 + 3795 WP_001144732.1 hypothetical protein -
IQS26_RS23540 84409..84825 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
IQS26_RS23545 84822..85052 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IQS26_RS23550 85317..85817 + 501 WP_000528932.1 hypothetical protein -
IQS26_RS23555 85830..86603 + 774 WP_000905949.1 hypothetical protein -
IQS26_RS23560 86770..87903 + 1134 WP_000545986.1 DUF3800 domain-containing protein -
IQS26_RS23565 87937..88449 - 513 WP_000151784.1 hypothetical protein -
IQS26_RS23570 88993..89211 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
IQS26_RS23575 89213..89518 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..169293 169293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T178296 WP_001034046.1 NZ_CP063462:c84825-84409 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T178296 NZ_CP083913:c198277-198005 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT178296 WP_001261278.1 NZ_CP063462:c85052-84822 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT178296 NZ_CP083913:c198540-198277 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References