178295

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 79546..80189 Replicon plasmid pEM22-18-53_3
Accession NZ_CP063462
Organism Escherichia coli strain EM22-18-53

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag IQS26_RS23525 Protein ID WP_001034044.1
Coordinates 79546..79962 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag IQS26_RS23530 Protein ID WP_001261286.1
Coordinates 79959..80189 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS26_RS23505 74678..75592 - 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -
IQS26_RS23510 75948..76645 + 698 Protein_65 IS1 family transposase -
IQS26_RS23515 76899..77921 - 1023 WP_000361402.1 helicase UvrD -
IQS26_RS23520 77906..79471 - 1566 WP_001128474.1 AAA family ATPase -
IQS26_RS23525 79546..79962 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
IQS26_RS23530 79959..80189 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IQS26_RS23535 80570..84364 + 3795 WP_001144732.1 hypothetical protein -
IQS26_RS23540 84409..84825 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
IQS26_RS23545 84822..85052 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA 1..169293 169293
- flank IS/Tn sitABCD - 72143..76645 4502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T178295 WP_001034044.1 NZ_CP063462:c79962-79546 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T178295 NZ_CP083913:47853-48203 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT178295 WP_001261286.1 NZ_CP063462:c80189-79959 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT178295 NZ_CP083913:47596-47859 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References