Detailed information of TA system TA178294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 11499..11875
• Replicon: plasmid pEM22-18-53_3
• Accession: NZ_CP063462
• Organism: Escherichia coli strain EM22-18-53

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: IQS26_RS23255
• Protein ID: WP_000969988.1
• Coordinates: 11693..11875 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: IQS26_RS23250
• Protein ID: WP_001324224.1
• Coordinates: 11499..11696 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS26_RS23230	6759..6995	+	237	WP_001323890.1	colicin V immunity protein	-
IQS26_RS23235	6973..7284	+	312	WP_001259758.1	colicin V	-
IQS26_RS23240	7454..9568	-	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
IQS26_RS23245	9543..10817	-	1275	WP_000489608.1	HlyD family secretion protein	-
IQS26_RS23250	11499..11696	+	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
IQS26_RS23255	11693..11875	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IQS26_RS23260	11974..12282	+	309	WP_074400545.1	hypothetical protein	-
IQS26_RS23265	12842..13875	+	1034	Protein_16	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..169293	169293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T178294 WP_000969988.1 NZ_CP063462:11693-11875 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T178294 NZ_CP083912:c2229263-2228922 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT178294 WP_001324224.1 NZ_CP063462:11499-11696 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT178294 NZ_CP083912:c2229493-2229263 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A075MDK3