Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4608590..4609208 Replicon chromosome
Accession NZ_CP063459
Organism Escherichia coli strain EM22-18-53

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IQS26_RS22080 Protein ID WP_001291435.1
Coordinates 4608990..4609208 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IQS26_RS22075 Protein ID WP_000344800.1
Coordinates 4608590..4608964 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS26_RS22065 4603679..4604872 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IQS26_RS22070 4604895..4608044 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IQS26_RS22075 4608590..4608964 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IQS26_RS22080 4608990..4609208 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IQS26_RS22085 4609380..4609931 + 552 WP_000102568.1 maltose O-acetyltransferase -
IQS26_RS22090 4610047..4610517 + 471 WP_000136192.1 YlaC family protein -
IQS26_RS22095 4610681..4612231 + 1551 WP_048943370.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IQS26_RS22100 4612273..4612626 - 354 Protein_4304 DUF1428 family protein -
IQS26_RS22110 4613005..4613316 + 312 WP_000409911.1 MGMT family protein -
IQS26_RS22115 4613347..4613919 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T178293 WP_001291435.1 NZ_CP063459:4608990-4609208 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T178293 NZ_CP083912:221905-222171 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT178293 WP_000344800.1 NZ_CP063459:4608590-4608964 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT178293 NZ_CP083912:221642-221905 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References