Detailed information of TA system TA178282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2463980..2464605
• Replicon: chromosome
• Accession: NZ_CP063459
• Organism: Escherichia coli strain EM22-18-53

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IQS26_RS11835
• Protein ID: WP_000911330.1
• Coordinates: 2464207..2464605 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IQS26_RS11830
• Protein ID: WP_000450524.1
• Coordinates: 2463980..2464207 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQS26_RS11805	2459783..2460253	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
IQS26_RS11810	2460253..2460825	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IQS26_RS11815	2460971..2461849	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IQS26_RS11820	2461866..2462900	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
IQS26_RS11825	2463113..2463826	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IQS26_RS11830	2463980..2464207	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IQS26_RS11835	2464207..2464605	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IQS26_RS11840	2464752..2465615	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
IQS26_RS11845	2465630..2467645	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
IQS26_RS11850	2467719..2468417	+	699	WP_000679823.1	esterase	-
IQS26_RS11855	2468527..2468727	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T178282 WP_000911330.1 NZ_CP063459:2464207-2464605 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T178282 NZ_CP083911:1568621-1569022 [Tepidimonas taiwanensis]
ATGATCCTGCTCGACACCAACATTTGCATTTACCTCATCAACCGCAAACCGCCCGCGGTGCTGCAGCGCTTCCGCGCCTA
CCGCGCCGGGGAGATCGGGCTGTCCTCCATCGTCGCCGCCGAGCTGGCCTACGGTGTCGCCAAAAGTGGATCGGCGCGCA
ACCAACAGGCGCTGGAACTGTTTCTCGCCCCGCTGGAGATCCACCCGTTCGACACACAGGCCATCTGGGTGTACGGCCGT
TTGCGCGCCACGCTCGAGCAACAGGGCACCCCGATCGGCGCGATGGACACCCTGATCGCCGCGCATGCCCTCGCGCTAGA
CGCCACCCTCGTCACCCACAACACGCGGGAGTTTGCCCGGGTCCCGGGCCTGCGGCTGGAGGATTGGGCGCTGGCCTCGT
GA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT178282 WP_000450524.1 NZ_CP063459:2463980-2464207 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT178282 NZ_CP083911:1568391-1568624 [Tepidimonas taiwanensis]
ATGGAAACCGCCAAGCTCTTCACCACGGGCCGCAGCCAAGCTGTCCGCCTGCCCAAACCCTACCGGTTCCATGGCCGTGA
AGTGGGCATACGCCGCGTGGGGGCCGGGATTCTGCTTTTTCCGGTCGACGACCCGTGGAGCGTGCTGGAACAGGCGCTCG
ACCTGTTCGAACCCGGGTTCGAGCTGAAGCGCGATCAACCCCCGCCACAAGCCCGGCCCGAGATTGCCGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33