Detailed information of TA system TA178271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3036448..3036964
• Replicon: chromosome
• Accession: NZ_CP063456
• Organism: Pseudomonas shirazica strain A28

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: IMX18_RS13770
• Protein ID: WP_217190849.1
• Coordinates: 3036448..3036729 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8G1E6
• Locus tag: IMX18_RS13775
• Protein ID: WP_013972043.1
• Coordinates: 3036719..3036964 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMX18_RS13745	3031523..3032764	-	1242	WP_217190848.1	Zn-dependent hydrolase	-
IMX18_RS13750	3032795..3034099	-	1305	WP_039613369.1	MFS transporter	-
IMX18_RS13755	3034391..3035299	+	909	WP_013972045.1	LysR family transcriptional regulator	-
IMX18_RS13760	3035388..3035783	+	396	WP_023662326.1	hypothetical protein	-
IMX18_RS13765	3035800..3036432	+	633	WP_015269938.1	BRCT domain-containing protein	-
IMX18_RS13770	3036448..3036729	-	282	WP_217190849.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IMX18_RS13775	3036719..3036964	-	246	WP_013972043.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IMX18_RS13780	3037208..3038314	-	1107	WP_217190850.1	alkene reductase	-
IMX18_RS13785	3038345..3039538	-	1194	WP_025338595.1	MFS transporter	-
IMX18_RS13795	3040274..3040816	+	543	WP_217190851.1	WYL domain-containing protein	-
IMX18_RS13800	3041073..3041552	+	480	WP_217190852.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10924.75 Da
• Isoelectric Point: 10.5787

>T178271 WP_217190849.1 NZ_CP063456:c3036729-3036448 [Pseudomonas shirazica]
MTYELEFLPSALKEWGKLGHTVREQIKKKLAERLQSPKVQADALRDLPNHYKIKLKASGYRLVYRVEEERIVVVVVSVGK
RERSEVYRSAQKR

Nucleotide

• Length: 282 bp

>T178271 NZ_CP083889:183863-184279 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8779.21 Da
• Isoelectric Point: 4.3611

>AT178271 WP_013972043.1 NZ_CP063456:c3036964-3036719 [Pseudomonas shirazica]
MQNIFADVAVSVSELKKNPSAVMTGAAGMPVAVLNHNRVMGYMVPAELYEAMMERLEDLELSELAKSRLGEKGVPVSLDD
L

Nucleotide

• Length: 246 bp

>AT178271 NZ_CP083889:183636-183866 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGAGCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: F8G1E6