Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 1367262..1368301 Replicon chromosome
Accession NZ_CP063456
Organism Pseudomonas shirazica strain A28

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A2N5BP56
Locus tag IMX18_RS06190 Protein ID WP_015271072.1
Coordinates 1367262..1367837 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2N5BP88
Locus tag IMX18_RS06195 Protein ID WP_015271071.1
Coordinates 1367834..1368301 (-) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMX18_RS06180 1362848..1363753 - 906 WP_013973410.1 LysR family transcriptional regulator -
IMX18_RS06185 1364117..1367047 - 2931 WP_217190185.1 aminotransferase -
IMX18_RS06190 1367262..1367837 - 576 WP_015271072.1 PIN domain-containing protein Toxin
IMX18_RS06195 1367834..1368301 - 468 WP_015271071.1 helix-turn-helix domain-containing protein Antitoxin
IMX18_RS06200 1368483..1368746 - 264 WP_015271070.1 DUF3077 domain-containing protein -
IMX18_RS06205 1368903..1369145 - 243 WP_015271069.1 hypothetical protein -
IMX18_RS06210 1369603..1371216 - 1614 WP_085720482.1 ABC transporter ATP-binding protein -
IMX18_RS06215 1371218..1372237 - 1020 WP_013973404.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1357817..1368301 10484


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

SinI-like, DNA-binding domain

(84-129)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21970.23 Da        Isoelectric Point: 5.0798

>T178268 WP_015271072.1 NZ_CP063456:c1367837-1367262 [Pseudomonas shirazica]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSTKIHDEWKRNLLLNRPDLTLEQVDRTSRRMDAAVPDALVTDY
ESLVEGLDLPDKDDRHVLAAAIKCNASVIVTFNLKDFPETALDVFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRMSLKN
PPHSVQQYLDRLLQQKLPESVKLLSEFKFLL

Download         Length: 576 bp

>T178268 NZ_CP083889:119823-120221 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17366.86 Da        Isoelectric Point: 5.3408

>AT178268 WP_015271071.1 NZ_CP063456:c1368301-1367834 [Pseudomonas shirazica]
MTTADLSRKMLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPYLVKLLDESVIPHTKTGRHRRVKFSDLVAYKDKRDEVSRAAMDALAAEAQELRMGYE

Download         Length: 468 bp

>AT178268 NZ_CP083889:119596-119823 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGATTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N5BP56

Antitoxin

Source ID Structure
AlphaFold DB A0A2N5BP88

References