Detailed information of TA system TA178256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 5162162..5162661
• Replicon: chromosome
• Accession: NZ_CP063450
• Organism: Rhodococcus pyridinivorans strain 5Ap

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V9XF56
• Locus tag: INP59_RS24100
• Protein ID: WP_024102500.1
• Coordinates: 5162162..5162416 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: INP59_RS24105
• Protein ID: WP_193902860.1
• Coordinates: 5162413..5162661 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INP59_RS24075	5158620..5158832	-	213	WP_193902856.1	hypothetical protein	-
INP59_RS24080	5159395..5160351	-	957	WP_193902857.1	Lsr2 family protein	-
INP59_RS24085	5160508..5161017	+	510	WP_193902858.1	hypothetical protein	-
INP59_RS24090	5161002..5161313	+	312	WP_193902859.1	plasmid pRiA4b ORF-3 family protein	-
INP59_RS24095	5161845..5162081	+	237	Protein_4749	hypothetical protein	-
INP59_RS24100	5162162..5162416	-	255	WP_024102500.1	Txe/YoeB family addiction module toxin	Toxin
INP59_RS24105	5162413..5162661	-	249	WP_193902860.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
INP59_RS24110	5162697..5163026	+	330	WP_193902861.1	hypothetical protein	-
INP59_RS24115	5163493..5164287	+	795	WP_006550857.1	LLM class F420-dependent oxidoreductase	-
INP59_RS24120	5164348..5165598	+	1251	WP_138845195.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5156917..5158314	1397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-70)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10077.42 Da
• Isoelectric Point: 8.0410

>T178256 WP_024102500.1 NZ_CP063450:c5162416-5162162 [Rhodococcus pyridinivorans]
VNITFTDEAWDDYQFWISTDRKQLKRVNELIRDCQRDPYSGIGKPERLRHALSGLWSRRIDKEHRLVYEVIDGALVIIAA
RYHY

Nucleotide

• Length: 255 bp

>T178256 NZ_CP083888:1324507-1324905 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9276.56 Da
• Isoelectric Point: 5.8315

>AT178256 WP_193902860.1 NZ_CP063450:c5162661-5162413 [Rhodococcus pyridinivorans]
MAAITATEARKNFFPLLEQVNKDHTPVEIVSKRGNAVLMSKDDYDAMLETSYLMQSPANARRLLASMQRARGGEYTEREL
VE

Nucleotide

• Length: 249 bp

>AT178256 NZ_CP083888:1324280-1324507 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: A0A7M2XPP3