Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 176729..177541 Replicon plasmid p2
Accession NZ_CP063447
Organism Novosphingobium sp. ES2-1

Toxin (Protein)


Gene name tacT Uniprot ID A0A7M2XF96
Locus tag IM701_RS18955 Protein ID WP_062343466.1
Coordinates 176729..177238 (-) Length 170 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A7M2XF82
Locus tag IM701_RS18960 Protein ID WP_062343467.1
Coordinates 177242..177541 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM701_RS18930 172288..172992 + 705 WP_193829014.1 DUF3800 domain-containing protein -
IM701_RS18935 173157..173847 + 691 Protein_167 pentapeptide repeat-containing protein -
IM701_RS18940 174367..174711 + 345 WP_193828878.1 thermonuclease family protein -
IM701_RS18945 175188..175523 + 336 WP_039336867.1 single-stranded DNA-binding protein -
IM701_RS18950 176030..176587 + 558 WP_193828879.1 hypothetical protein -
IM701_RS18955 176729..177238 - 510 WP_062343466.1 hypothetical protein Toxin
IM701_RS18960 177242..177541 - 300 WP_062343467.1 DUF1778 domain-containing protein Antitoxin
IM701_RS18965 177617..177979 + 363 WP_193828880.1 XRE family transcriptional regulator -
IM701_RS18970 178230..178709 - 480 WP_193828881.1 hypothetical protein -
IM701_RS18975 178706..180829 - 2124 WP_193828882.1 tyrosine-type recombinase/integrase -
IM701_RS18980 180820..181986 - 1167 WP_193829015.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fcl / gmd 1..336628 336628


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(3-92)


Sequences

Toxin        


Download         Length: 170 a.a.        Molecular weight: 17739.49 Da        Isoelectric Point: 6.4739

>T178252 WP_062343466.1 NZ_CP063447:c177238-176729 [Novosphingobium sp. ES2-1]
MGLSAPELLNAAHEVDDFDCGKPSLNHWLKGRALANQERGFTAVLVVHEAMRVVGYYGLAPTSVIPGILPRSIRTGQPPD
PVPCLLLGQLATDLAWAGQGIGSGLLRHALGRCLEAARLVGGRAVIVNAIDDEAAAFWLGKGFLPSRDDPLVLFQSIGAI
AASLAAAER

Download         Length: 510 bp

>T178252 NZ_CP083888:721792-722088 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11137.79 Da        Isoelectric Point: 5.7456

>AT178252 WP_062343467.1 NZ_CP063447:c177541-177242 [Novosphingobium sp. ES2-1]
MAKSLERKDHPVSMRLPDADIALIDRAAIARGRSRTDFVREAAVRAAEDVLMDQRLVRMSTEGFAEFIALLEKPAEAVPS
LVDVLRRPAPWDMPSDDRH

Download         Length: 300 bp

>AT178252 NZ_CP083888:722090-722485 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7M2XF96

Antitoxin

Source ID Structure
AlphaFold DB A0A7M2XF82

References