Detailed information of TA system TA178250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3990014..3990530
• Replicon: chromosome
• Accession: NZ_CP063441
• Organism: Enterobacter asburiae strain SD4L

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: INS04_RS18715
• Protein ID: WP_045402335.1
• Coordinates: 3990014..3990298 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V3EXX3
• Locus tag: INS04_RS18720
• Protein ID: WP_008501400.1
• Coordinates: 3990288..3990530 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INS04_RS18700	3985229..3986872	+	1644	WP_193805152.1	alpha,alpha-phosphotrehalase	-
INS04_RS18705	3987248..3989386	+	2139	WP_032662478.1	anaerobic ribonucleoside-triphosphate reductase	-
INS04_RS18710	3989546..3990010	+	465	WP_063931015.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
INS04_RS18715	3990014..3990298	-	285	WP_045402335.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INS04_RS18720	3990288..3990530	-	243	WP_008501400.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
INS04_RS18725	3990608..3992518	-	1911	WP_193805153.1	BglG family transcription antiterminator	-
INS04_RS18730	3992538..3993674	-	1137	WP_193805154.1	lactonase family protein	-
INS04_RS18735	3993711..3994451	-	741	WP_193805155.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10907.73 Da
• Isoelectric Point: 10.3350

>T178250 WP_045402335.1 NZ_CP063441:c3990298-3990014 [Enterobacter asburiae]
MTYELAFDPRALKEWSKLGQTVKDQFKKKLADVLVNPRVESARLHGLPDCYKIKLRTQGYRLVYQVQDNVVTVFVIAIGK
REKSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T178250 NZ_CP083888:263780-264010 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8972.37 Da
• Isoelectric Point: 5.0651

>AT178250 WP_008501400.1 NZ_CP063441:c3990530-3990288 [Enterobacter asburiae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT178250 NZ_CP083888:264007-264366 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: V3EXX3