Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3462402..3463022 Replicon chromosome
Accession NZ_CP063441
Organism Enterobacter asburiae strain SD4L

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag INS04_RS16275 Protein ID WP_008499287.1
Coordinates 3462804..3463022 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag INS04_RS16270 Protein ID WP_008499288.1
Coordinates 3462402..3462776 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INS04_RS16260 3457528..3458721 + 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
INS04_RS16265 3458744..3461890 + 3147 WP_047650452.1 multidrug efflux RND transporter permease subunit AcrB -
INS04_RS16270 3462402..3462776 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
INS04_RS16275 3462804..3463022 + 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
INS04_RS16280 3463228..3463779 + 552 WP_193804924.1 maltose O-acetyltransferase -
INS04_RS16285 3463897..3464364 + 468 WP_023310598.1 hypothetical protein -
INS04_RS16290 3464336..3465796 - 1461 WP_193804925.1 PLP-dependent aminotransferase family protein -
INS04_RS16295 3465898..3466608 + 711 WP_193804926.1 GNAT family N-acetyltransferase -
INS04_RS16300 3466605..3466745 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
INS04_RS16305 3466748..3467008 - 261 WP_006176933.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T178249 WP_008499287.1 NZ_CP063441:3462804-3463022 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T178249 NZ_CP083886:c4020340-4020053 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT178249 WP_008499288.1 NZ_CP063441:3462402-3462776 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT178249 NZ_CP083886:c4020066-4019764 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References