Detailed information of TA system TA178235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3741432..3742081
• Replicon: chromosome
• Accession: NZ_CP063440
• Organism: Proteus mirabilis strain Yak 2019

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B4EZB9
• Locus tag: INT85_RS17315
• Protein ID: WP_012368534.1
• Coordinates: 3741432..3741851 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: INT85_RS17320
• Protein ID: WP_012368535.1
• Coordinates: 3741848..3742081 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INT85_RS17285	3736709..3737587	-	879	WP_049203255.1	23S rRNA pseudouridine(2604) synthase RluF	-
INT85_RS17290	3737763..3738677	-	915	WP_004249085.1	fatty acid biosynthesis protein FabY	-
INT85_RS17295	3738701..3739138	-	438	WP_004246810.1	D-tyrosyl-tRNA(Tyr) deacylase	-
INT85_RS17300	3739219..3739836	-	618	WP_004246809.1	glucose-1-phosphatase	-
INT85_RS17305	3740490..3740906	-	417	WP_049208252.1	hypothetical protein	-
INT85_RS17310	3740884..3741207	-	324	WP_049208253.1	hypothetical protein	-
INT85_RS17315	3741432..3741851	-	420	WP_012368534.1	type II toxin-antitoxin system VapC family toxin	Toxin
INT85_RS17320	3741848..3742081	-	234	WP_012368535.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
INT85_RS17325	3742833..3744668	-	1836	WP_004246802.1	ribosome-dependent GTPase TypA	-
INT85_RS17330	3745028..3746437	+	1410	WP_004246801.1	glutamate--ammonia ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15371.88 Da
• Isoelectric Point: 7.7288

>T178235 WP_012368534.1 NZ_CP063440:c3741851-3741432 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISAITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T178235 NZ_CP083886:716866-717162 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT178235 WP_012368535.1 NZ_CP063440:c3742081-3741848 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT178235 NZ_CP083886:717164-717559 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: B4EZB9

Antitoxin

• AlphaFold DB: B4EZC0