Detailed information of TA system TA178234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 3720860..3721559
• Replicon: chromosome
• Accession: NZ_CP063440
• Organism: Proteus mirabilis strain Yak 2019

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: INT85_RS17210
• Protein ID: WP_004249093.1
• Coordinates: 3720860..3721246 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: INT85_RS17215
• Protein ID: WP_004246828.1
• Coordinates: 3721239..3721559 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INT85_RS17195	3716879..3717460	-	582	WP_004246833.1	DNA-3-methyladenine glycosylase I	-
INT85_RS17200	3717711..3718616	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
INT85_RS17205	3718626..3720698	+	2073	WP_004246830.1	glycine--tRNA ligase subunit beta	-
INT85_RS17210	3720860..3721246	+	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INT85_RS17215	3721239..3721559	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
INT85_RS17220	3721608..3722042	-	435	WP_004246827.1	hypothetical protein	-
INT85_RS17225	3722035..3722919	-	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
INT85_RS17230	3723136..3724422	-	1287	WP_049196833.1	DUF3748 domain-containing protein	-
INT85_RS17235	3724559..3725176	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
INT85_RS17240	3725478..3726101	+	624	WP_004246821.1	guanylate kinase	-
INT85_RS17245	3726156..3726431	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T178234 WP_004249093.1 NZ_CP063440:3720860-3721246 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T178234 NZ_CP083886:651161-651475 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT178234 WP_004246828.1 NZ_CP063440:3721239-3721559 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT178234 NZ_CP083886:651472-651888 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: B4EZ96

Antitoxin

• AlphaFold DB: B4EZ97