Detailed information of TA system TA178233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3352237..3353959
• Replicon: chromosome
• Accession: NZ_CP063440
• Organism: Proteus mirabilis strain Yak 2019

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A0U2S2I2
• Locus tag: INT85_RS15590
• Protein ID: WP_064497040.1
• Coordinates: 3352237..3353175 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A934MVJ4
• Locus tag: INT85_RS15595
• Protein ID: WP_000095577.1
• Coordinates: 3353168..3353959 (-)
• Length: 264 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INT85_RS15570	3348178..3348690	-	513	WP_175215521.1	S26 family signal peptidase	-
INT85_RS15575	3348674..3349021	-	348	WP_064497038.1	hypothetical protein	-
INT85_RS15580	3349014..3351413	-	2400	WP_064497039.1	type IV secretion system protein TraC	-
INT85_RS15585	3351413..3352105	-	693	WP_001228924.1	DsbC family protein	-
INT85_RS15590	3352237..3353175	-	939	WP_064497040.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
INT85_RS15595	3353168..3353959	-	792	WP_000095577.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
INT85_RS15600	3354180..3354566	-	387	WP_032469918.1	hypothetical protein	-
INT85_RS15605	3354563..3355135	-	573	WP_024008834.1	type IV conjugative transfer system lipoprotein TraV	-
INT85_RS15610	3355210..3356499	-	1290	WP_064497041.1	TraB/VirB10 family protein	-
INT85_RS15615	3356502..3357398	-	897	WP_064497055.1	type-F conjugative transfer system secretin TraK	-
INT85_RS15620	3357382..3358008	-	627	WP_064497042.1	pilus assembly protein	-
INT85_RS15625	3358005..3358286	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(J) / aadA2 / ere(A) / dfrA32 / floR / sul2 / aph(4)-Ia / aac(3)-IVa / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / blaCTX-M-65 / fosA3 / tet(C) / aph(3'')-Ib / aph(6)-Id	-	3207187..3432374	225187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-272)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (99-250)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 35542.81 Da
• Isoelectric Point: 4.9912

>T178233 WP_064497040.1 NZ_CP063440:c3353175-3352237 [Proteus mirabilis]
MDKDSPYYKQVSLLIRMLPVVATETVFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPRDEALINVRAALQRITDRINTQ
PDIRAAFQDNKADELRIIVSSPVATIKIEVSPVARGTLHSAEIMAVQESVEYEFGYAEIQVVSLPDLYGGKLCAAMDRQH
PRDLFDVRMLLGSEGVSREILVGFLTYTLSHPRPINEVMSPNWQPLNEKFQAEFDGMTFEKVECEDLASVRPMMLTSLQK
HFTERDYAFLMSFKRGQPDWALFDYPNAADLPAIRWKLQNINKLAKNQAKHQEQLDKLKQVLDDWLVNANAE

Nucleotide

• Length: 939 bp

>T178233 NZ_CP083886:c608288-607824 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 264 a.a.
• Molecular weight: 30109.49 Da
• Isoelectric Point: 9.5097

>AT178233 WP_000095577.1 NZ_CP063440:c3353959-3353168 [Proteus mirabilis]
MSSKINWLVAHTSPGALVLQQWLTENGVSYSLAQKYAQNGWLKKLSSGVYYRPNAQGDIKPTWVDAIQALDVQLGVPVHL
AGLSSLTHQGLSHYLQLNKEQVWICVKNKSSLPKWFREFPYQNWFYCGNHKLEVNPEKDLKRITVKEKELTVSCAELAAY
EVVDAIGKLISFEHVAELFQGLVNLSPRKVQDILERSSSVQANRIFLFLGRYYDHQWVNRIDETRIKLGAGKRQVVEKGR
FDERYQITVPEILSVKKGEQHNG

Nucleotide

• Length: 792 bp

>AT178233 NZ_CP083886:c608623-608288 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0U2S2I2