Detailed information of TA system TA178228

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4421761..4422424
• Replicon: chromosome
• Accession: NZ_CP063427
• Organism: Citrobacter sp. BDA59-3

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A348D8P9
• Locus tag: IP582_RS20775
• Protein ID: WP_039077972.1
• Coordinates: 4421761..4422177 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: IP582_RS20780
• Protein ID: WP_193794326.1
• Coordinates: 4422158..4422424 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IP582_RS20755	4417763..4419496	-	1734	WP_193794322.1	single-stranded-DNA-specific exonuclease RecJ	-
IP582_RS20760	4419502..4420215	-	714	WP_193794323.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
IP582_RS20765	4420239..4421135	-	897	WP_193794324.1	site-specific tyrosine recombinase XerD	-
IP582_RS20770	4421237..4421758	+	522	WP_193794325.1	flavodoxin FldB	-
IP582_RS20775	4421761..4422177	-	417	WP_039077972.1	protein YgfX	Toxin
IP582_RS20780	4422158..4422424	-	267	WP_193794326.1	FAD assembly factor SdhE	Antitoxin
IP582_RS20785	4422674..4423657	+	984	WP_193794327.1	tRNA-modifying protein YgfZ	-
IP582_RS20790	4423775..4424434	-	660	WP_039077975.1	hemolysin III family protein	-
IP582_RS20795	4424597..4424908	-	312	WP_193794328.1	N(4)-acetylcytidine aminohydrolase	-
IP582_RS20800	4424961..4425689	+	729	WP_039077977.1	MurR/RpiR family transcriptional regulator	-
IP582_RS20805	4425812..4427245	+	1434	WP_193794329.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16438.33 Da
• Isoelectric Point: 11.4778

>T178228 WP_039077972.1 NZ_CP063427:c4422177-4421761 [Citrobacter sp. BDA59-3]
VVLWQSDIRVSWRSQWLSLLLHGLMAAFVLLLPWPLSYTPLWLLLLSLVVFDSVRSQRRINACQGEIKLLMDARLRWQGV
EWEIVGTPWMLNIGMILRLRREKDGRRQHLWLAADSMDAQEWRDLRRLISQQSAQRQH

Nucleotide

• Length: 417 bp

>T178228 NZ_CP083884:c130248-129832 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10535.98 Da
• Isoelectric Point: 6.0701

>AT178228 WP_193794326.1 NZ_CP063427:c4422424-4422158 [Citrobacter sp. BDA59-3]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDDKHVFVRLLQSDDPDLFNWLMNHGKPQDEALQRMIQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT178228 NZ_CP083884:c130475-130245 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGAGCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A348D8P9