Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3456910..3457500 Replicon chromosome
Accession NZ_CP063427
Organism Citrobacter sp. BDA59-3

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag IP582_RS16380 Protein ID WP_107224096.1
Coordinates 3456910..3457242 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A7M2V158
Locus tag IP582_RS16385 Protein ID WP_041851870.1
Coordinates 3457243..3457500 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IP582_RS16355 3452618..3453892 + 1275 WP_071196351.1 extracellular solute-binding protein -
IP582_RS16360 3453911..3454822 + 912 WP_107224098.1 sugar ABC transporter permease -
IP582_RS16370 3455076..3456509 - 1434 WP_107224097.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
IP582_RS16380 3456910..3457242 - 333 WP_107224096.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
IP582_RS16385 3457243..3457500 - 258 WP_041851870.1 antitoxin Antitoxin
IP582_RS16390 3457925..3458836 - 912 WP_107224095.1 LysR family transcriptional regulator -
IP582_RS16395 3458937..3460046 + 1110 WP_193793824.1 alkene reductase -
IP582_RS16400 3460131..3460274 + 144 WP_193793825.1 hypothetical protein -
IP582_RS16405 3460346..3460885 + 540 WP_193793826.1 ester cyclase -
IP582_RS16410 3461144..3461434 + 291 WP_193793827.1 DUF3601 domain-containing protein -
IP582_RS16415 3461562..3461897 + 336 WP_041851877.1 gamma-glutamylcyclotransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(5-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11709.43 Da        Isoelectric Point: 8.0290

>T178227 WP_107224096.1 NZ_CP063427:c3457242-3456910 [Citrobacter sp. BDA59-3]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSPASFNALTRLPVVVPVTSGGNFARAAGFAVSLDGAGTKTTGVIRCDQPRTI
DMDARNGKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T178227 NZ_CP083884:41644-41793 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9286.76 Da        Isoelectric Point: 4.5799

>AT178227 WP_041851870.1 NZ_CP063427:c3457500-3457243 [Citrobacter sp. BDA59-3]
MYIARLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIENGCLIIEPQKRPHYSLEELLAQCDPHAEISEEDRQWIDAPAA
GKELL

Download         Length: 258 bp

>AT178227 NZ_CP083884:c41600-41539 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7M2V158

References