Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 2575092..2575714 Replicon chromosome
Accession NZ_CP063427
Organism Citrobacter sp. BDA59-3

Toxin (Protein)


Gene name hicA Uniprot ID A0A7M2VEM2
Locus tag IP582_RS12105 Protein ID WP_039079130.1
Coordinates 2575092..2575274 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7M2VD87
Locus tag IP582_RS12110 Protein ID WP_039079129.1
Coordinates 2575286..2575714 (+) Length 143 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IP582_RS12090 2571659..2571934 + 276 WP_039079133.1 metal/formaldehyde-sensitive transcriptional repressor -
IP582_RS12095 2571967..2573085 + 1119 WP_193796095.1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
IP582_RS12100 2573240..2574937 + 1698 WP_193796096.1 Tar ligand binding domain-containing protein -
IP582_RS12105 2575092..2575274 + 183 WP_039079130.1 type II toxin-antitoxin system HicA family toxin Toxin
IP582_RS12110 2575286..2575714 + 429 WP_039079129.1 hypothetical protein Antitoxin
IP582_RS12115 2575756..2578137 - 2382 WP_193796097.1 glucose/quinate/shikimate family membrane-bound PQQ-dependent dehydrogenase -
IP582_RS12120 2578395..2580692 - 2298 WP_193796098.1 FdhF/YdeP family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-82)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 7143.27 Da        Isoelectric Point: 10.5371

>T178225 WP_039079130.1 NZ_CP063427:2575092-2575274 [Citrobacter sp. BDA59-3]
MKYSEFRRWLLEQGAILQKHRRGSSHFKVYLDDRQSIFPDHGAKEMPEGTRKKIIKDLGL

Download         Length: 183 bp

>T178225 NZ_CP083882:21024-21275 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGACTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 143 a.a.        Molecular weight: 15785.29 Da        Isoelectric Point: 4.7105

>AT178225 WP_039079129.1 NZ_CP063427:2575286-2575714 [Citrobacter sp. BDA59-3]
MFNYAVKLTQDAETRVYEVSCRDLPLMHSVGDTPEEALLEAEDGIVTAISLEMDMRRPIPVATPAREDERVVIIPVLVAM
KAALYNAMIETGTRKADLARRLGVNAPQVDRLLDVEHSSKVEKVEFALAQLGRKIQVEIQIA

Download         Length: 429 bp

>AT178225 NZ_CP083882:21262-21489 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGCAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7M2VEM2

Antitoxin

Source ID Structure
AlphaFold DB A0A7M2VD87

References