Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1183832..1184451 Replicon chromosome
Accession NZ_CP063427
Organism Citrobacter sp. BDA59-3

Toxin (Protein)


Gene name Hha Uniprot ID A0A348DII6
Locus tag IP582_RS05480 Protein ID WP_041852465.1
Coordinates 1183832..1184050 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A348DII5
Locus tag IP582_RS05485 Protein ID WP_041852464.1
Coordinates 1184077..1184451 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IP582_RS05450 1180005..1180265 + 261 WP_041852470.1 type B 50S ribosomal protein L31 -
IP582_RS05455 1180269..1180409 + 141 WP_006818844.1 type B 50S ribosomal protein L36 -
IP582_RS05460 1180406..1181107 - 702 WP_041852469.1 GNAT family N-acetyltransferase -
IP582_RS05465 1181207..1182652 + 1446 WP_193795305.1 PLP-dependent aminotransferase family protein -
IP582_RS05470 1182630..1183097 - 468 WP_041852467.1 membrane protein -
IP582_RS05475 1183197..1183763 - 567 WP_193795306.1 maltose O-acetyltransferase -
IP582_RS05480 1183832..1184050 - 219 WP_041852465.1 hemolysin expression modulator Hha Toxin
IP582_RS05485 1184077..1184451 - 375 WP_041852464.1 Hha toxicity modulator TomB Antitoxin
IP582_RS05490 1184972..1188121 - 3150 WP_193795307.1 multidrug efflux RND transporter permease subunit AcrB -
IP582_RS05495 1188144..1189340 - 1197 WP_193795308.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8685.14 Da        Isoelectric Point: 8.9039

>T178216 WP_041852465.1 NZ_CP063427:c1184050-1183832 [Citrobacter sp. BDA59-3]
MSDKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELPDHELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T178216 NZ_CP083880:3443932-3444150 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14600.44 Da        Isoelectric Point: 5.5072

>AT178216 WP_041852464.1 NZ_CP063427:c1184451-1184077 [Citrobacter sp. BDA59-3]
MDEYSPKRHDIAQLKFLCETLYHDCLLNLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSKANPVSHSC

Download         Length: 375 bp

>AT178216 NZ_CP083880:3443532-3443906 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348DII6

Antitoxin

Source ID Structure
AlphaFold DB A0A348DII5

References