Detailed information of TA system TA178213

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4030488..4031151
• Replicon: chromosome
• Accession: NZ_CP063425
• Organism: Kosakonia pseudosacchari strain BDA62-3

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: IP581_RS19025
• Protein ID: WP_086873527.1
• Coordinates: 4030488..4030904 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1G4Y1L0
• Locus tag: IP581_RS19030
• Protein ID: WP_017457616.1
• Coordinates: 4030885..4031151 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IP581_RS19005	4026481..4028214	-	1734	WP_193821130.1	single-stranded-DNA-specific exonuclease RecJ	-
IP581_RS19010	4028220..4028933	-	714	WP_193821131.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
IP581_RS19015	4028958..4029854	-	897	WP_193821132.1	site-specific tyrosine recombinase XerD	-
IP581_RS19020	4029954..4030475	+	522	WP_086873528.1	flavodoxin FldB	-
IP581_RS19025	4030488..4030904	-	417	WP_086873527.1	protein YgfX	Toxin
IP581_RS19030	4030885..4031151	-	267	WP_017457616.1	FAD assembly factor SdhE	Antitoxin
IP581_RS19035	4031398..4032381	+	984	WP_097400859.1	tRNA-modifying protein YgfZ	-
IP581_RS19040	4032443..4033102	-	660	WP_193821133.1	hemolysin III family protein	-
IP581_RS19045	4033342..4034070	+	729	WP_086873857.1	MurR/RpiR family transcriptional regulator	-
IP581_RS19050	4034187..4035620	+	1434	WP_193821134.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16374.29 Da
• Isoelectric Point: 11.0079

>T178213 WP_086873527.1 NZ_CP063425:c4030904-4030488 [Kosakonia pseudosacchari]
VVLWQSDLRVSWRAQWISLLLHGLVAAFILFMPWPLSYTPLWLLLLSLVVFDCVRSQRRINACHGEIKLLMDSRLRWQGV
EWDIIGTPWMIRGGMMLRLRRAEDGRRQHLWLAADSMDSQEWRDLRRMITQNPAQGLH

Nucleotide

• Length: 417 bp

>T178213 NZ_CP083880:c2405584-2405408 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10521.95 Da
• Isoelectric Point: 6.0701

>AT178213 WP_017457616.1 NZ_CP063425:c4031151-4030885 [Kosakonia pseudosacchari]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDHDKQVFVRLLESDDPDLFNWLMNHGKPQDAELQRMVQLIQTRN
RDRGPVAI

Nucleotide

• Length: 267 bp

>AT178213 NZ_CP083880:c2405362-2404946 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1G4Y1L0