Detailed information of TA system TA178199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2027427..2029084
• Replicon: chromosome
• Accession: NZ_CP063424
• Organism: Actinobacillus pleuropneumoniae strain APP5

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A3N2X8
• Locus tag: IOD66_RS09470
• Protein ID: WP_011848633.1
• Coordinates: 2027427..2028758 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A3N2X7
• Locus tag: IOD66_RS09475
• Protein ID: WP_005613259.1
• Coordinates: 2028755..2029084 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD66_RS09465 (IOD66_09480)	2025404..2027095	+	1692	WP_042655118.1	AIPR family protein	-
IOD66_RS09470 (IOD66_09485)	2027427..2028758	+	1332	WP_011848633.1	HipA domain-containing protein	Toxin
IOD66_RS09475 (IOD66_09490)	2028755..2029084	+	330	WP_005613259.1	helix-turn-helix domain-containing protein	Antitoxin
IOD66_RS09480 (IOD66_09495)	2029128..2029637	-	510	WP_009874970.1	ferredoxin-type protein NapF	-
IOD66_RS09485 (IOD66_09500)	2029641..2030246	-	606	WP_009874971.1	Tat proofreading chaperone DmsD	-
IOD66_RS09490 (IOD66_09505)	2030302..2031135	-	834	WP_009874972.1	dimethyl sulfoxide reductase anchor subunit	-
IOD66_RS09495 (IOD66_09510)	2031137..2031754	-	618	WP_009874973.1	dimethylsulfoxide reductase subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (164-371)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-94)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51131.07 Da
• Isoelectric Point: 5.0347

>T178199 WP_011848633.1 NZ_CP063424:2027427-2028758 [Actinobacillus pleuropneumoniae]
MSDISVKNKKLTLQTYVDNLWRDMAELYFNAQYELESMEYLTDYAVDFFGQANARSISINYPVNVFIDHADKLMTTLADI
IPAGASRRYWLEALDIKHLSISHQNYLLLKNGAISPIGNVRVKEAVPEIQSERYFPLNVVQNRQSDFLEYANQTGAMVGG
ATGAGGEAPKLLLKCQDKQVWIDNLQLGLDCDTAYLVKYPRGNYSELDCDILRAEYHYYHELRALGFSTIDTDLMKLEEG
ERYPSLWLPRFDMKFKDGKWERYAMESVYSMLQKYGGNLDHEQTIRNLLNIISASQFSNDFDNRHFVIEWVKRDLLNIAF
GNSDNHGRNTAFLRDDNRIWLSPIYDFAPMRADPEGIIRTTTWAKNGDNPQEFAGEYRFDKIADGLSDLIEPDILLRELR
MLATQLVDLKSRLMKRGVPKSILDFPAIGFDYLPEKLQRWGLV

Nucleotide

• Length: 1332 bp

>T178199 NZ_CP083877:c4254044-4253871 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12320.45 Da
• Isoelectric Point: 10.8292

>AT178199 WP_005613259.1 NZ_CP063424:2028755-2029084 [Actinobacillus pleuropneumoniae]
MTIQHKNASPTRSKVNGIERQLIRETIIVQLLKGEISQGQALRRLRVEALGINQQDYLKLAKVSRQTLSNIENDKGNYSI
ETINQVFKPMGLKLGLMPISKDLMASFLK

Nucleotide

• Length: 330 bp

>AT178199 NZ_CP083877:c4254368-4254072 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A3N2X8

Antitoxin

• AlphaFold DB: A3N2X7