Detailed information of TA system TA178198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1965766..1966394
• Replicon: chromosome
• Accession: NZ_CP063424
• Organism: Actinobacillus pleuropneumoniae strain APP5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IOD66_RS09200
• Protein ID: WP_009875032.1
• Coordinates: 1965766..1966164 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3GYV7
• Locus tag: IOD66_RS09205
• Protein ID: WP_005613300.1
• Coordinates: 1966164..1966394 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD66_RS09175 (IOD66_09190)	1961389..1961910	+	522	WP_005618211.1	ATP-dependent protease subunit HslV	-
IOD66_RS09180 (IOD66_09195)	1961965..1963287	+	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
IOD66_RS09185 (IOD66_09200)	1963360..1964139	+	780	WP_005602546.1	hypothetical protein	-
IOD66_RS09190 (IOD66_09205)	1964319..1964969	-	651	WP_005618207.1	DUF2202 domain-containing protein	-
IOD66_RS09195 (IOD66_09210)	1965215..1965631	+	417	WP_005618204.1	DUF417 family protein	-
IOD66_RS09200 (IOD66_09215)	1965766..1966164	-	399	WP_009875032.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IOD66_RS09205 (IOD66_09220)	1966164..1966394	-	231	WP_005613300.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IOD66_RS09210 (IOD66_09225)	1966916..1968547	-	1632	WP_042655123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15105.39 Da
• Isoelectric Point: 7.4195

>T178198 WP_009875032.1 NZ_CP063424:c1966164-1965766 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCISSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T178198 NZ_CP083877:c4151875-4151525 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8625.71 Da
• Isoelectric Point: 4.5358

>AT178198 WP_005613300.1 NZ_CP063424:c1966394-1966164 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT178198 NZ_CP083877:c4152120-4151869 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A3N328