Detailed information of TA system TA178197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 1881799..1882514
• Replicon: chromosome
• Accession: NZ_CP063424
• Organism: Actinobacillus pleuropneumoniae strain APP5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IOD66_RS08680
• Protein ID: WP_005599445.1
• Coordinates: 1882149..1882514 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: IOD66_RS08675
• Protein ID: WP_005599447.1
• Coordinates: 1881799..1882086 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD66_RS08650 (IOD66_08670)	1877402..1877995	-	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-
IOD66_RS08655 (IOD66_08675)	1877995..1878951	-	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
IOD66_RS08660 (IOD66_08680)	1879040..1879621	+	582	WP_207618149.1	sigma-70 family RNA polymerase sigma factor	-
IOD66_RS08665	1879624..1879812	+	189	WP_005602684.1	hypothetical protein	-
IOD66_RS08670 (IOD66_08685)	1879878..1881593	-	1716	WP_009875324.1	proline--tRNA ligase	-
IOD66_RS08675 (IOD66_08690)	1881799..1882086	+	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IOD66_RS08680 (IOD66_08695)	1882149..1882514	+	366	WP_005599445.1	type II toxin-antitoxin system VapC family toxin	Toxin
IOD66_RS08685 (IOD66_08700)	1882638..1884719	+	2082	WP_009875323.1	ATP-dependent DNA helicase RecG	-
IOD66_RS08690 (IOD66_08705)	1884846..1885127	+	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
IOD66_RS08695 (IOD66_08710)	1885223..1886929	+	1707	WP_005609259.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13974.06 Da
• Isoelectric Point: 9.0549

>T178197 WP_005599445.1 NZ_CP063424:1882149-1882514 [Actinobacillus pleuropneumoniae]
MDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T178197 NZ_CP083877:c4041381-4041094 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT178197 WP_005599447.1 NZ_CP063424:1881799-1882086 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT178197 NZ_CP083877:c4041107-4040805 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5