Detailed information of TA system TA178196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1825719..1826450
• Replicon: chromosome
• Accession: NZ_CP063424
• Organism: Actinobacillus pleuropneumoniae strain APP5

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A3N2I6
• Locus tag: IOD66_RS08420
• Protein ID: WP_009875245.1
• Coordinates: 1825719..1826186 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B0BRD3
• Locus tag: IOD66_RS08425
• Protein ID: WP_005619269.1
• Coordinates: 1826190..1826450 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD66_RS08395 (IOD66_08415)	1821077..1821448	-	372	WP_005598830.1	CrcB family protein	-
IOD66_RS08400 (IOD66_08420)	1821451..1822515	-	1065	WP_011848610.1	MFS transporter	-
IOD66_RS08405 (IOD66_08425)	1822610..1823719	+	1110	WP_009875243.1	anhydro-N-acetylmuramic acid kinase	-
IOD66_RS08410 (IOD66_08430)	1823773..1824687	+	915	WP_009875244.1	N-acetylmuramic acid 6-phosphate etherase	-
IOD66_RS08415 (IOD66_08435)	1824754..1825635	-	882	WP_005608878.1	50S ribosomal protein L11 methyltransferase	-
IOD66_RS08420 (IOD66_08440)	1825719..1826186	-	468	WP_009875245.1	GNAT family N-acetyltransferase	Toxin
IOD66_RS08425 (IOD66_08445)	1826190..1826450	-	261	WP_005619269.1	DUF1778 domain-containing protein	Antitoxin
IOD66_RS08430 (IOD66_08450)	1826571..1828031	-	1461	WP_009875246.1	metalloprotease TldD	-
IOD66_RS08435 (IOD66_08455)	1828161..1829420	+	1260	WP_005608887.1	tRNA lysidine(34) synthetase TilS	-
IOD66_RS08440 (IOD66_08460)	1829447..1829659	-	213	WP_237593654.1	DUF5389 family protein	-
IOD66_RS08445 (IOD66_08465)	1829739..1830632	-	894	WP_005598850.1	site-specific tyrosine recombinase XerD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (40-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17227.20 Da
• Isoelectric Point: 9.5125

>T178196 WP_009875245.1 NZ_CP063424:c1826186-1825719 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVRDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNPQAKQFYLKYGFSCSPIDEIVLMLKL

Nucleotide

• Length: 468 bp

>T178196 NZ_CP083877:3704790-3705068 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9796.14 Da
• Isoelectric Point: 4.4883

>AT178196 WP_005619269.1 NZ_CP063424:c1826450-1826190 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Nucleotide

• Length: 261 bp

>AT178196 NZ_CP083877:3704527-3704787 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A3N2I6

Antitoxin

• AlphaFold DB: A3N2I7