Detailed information of TA system TA178195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 1005902..1006552
• Replicon: chromosome
• Accession: NZ_CP063424
• Organism: Actinobacillus pleuropneumoniae strain APP5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: IOD66_RS04700
• Protein ID: WP_005614926.1
• Coordinates: 1006163..1006552 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: IOD66_RS04695
• Protein ID: WP_005604117.1
• Coordinates: 1005902..1006162 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD66_RS04675 (IOD66_04690)	1000922..1001542	-	621	WP_009874703.1	hypothetical protein	-
IOD66_RS04680 (IOD66_04695)	1001553..1002746	-	1194	WP_009874702.1	iron uptake transporter deferrochelatase/peroxidase subunit	-
IOD66_RS04685 (IOD66_04700)	1002758..1003570	-	813	WP_005607533.1	iron uptake system protein EfeO	-
IOD66_RS04690 (IOD66_04705)	1003747..1005768	+	2022	WP_009874701.1	excinuclease ABC subunit UvrB	-
IOD66_RS04695 (IOD66_04710)	1005902..1006162	+	261	WP_005604117.1	hypothetical protein	Antitoxin
IOD66_RS04700 (IOD66_04715)	1006163..1006552	+	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
IOD66_RS04705 (IOD66_04720)	1006688..1007911	+	1224	WP_009874700.1	ATP-binding protein	-
IOD66_RS04710 (IOD66_04725)	1008320..1009606	+	1287	WP_009874699.1	Xaa-Pro aminopeptidase	-
IOD66_RS04715 (IOD66_04730)	1009785..1010981	-	1197	WP_009874698.1	amino acid aminotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T178195 WP_005614926.1 NZ_CP063424:1006163-1006552 [Actinobacillus pleuropneumoniae]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T178195 NZ_CP083877:c3698728-3698330 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT178195 WP_005604117.1 NZ_CP063424:1005902-1006162 [Actinobacillus pleuropneumoniae]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT178195 NZ_CP083877:c3699024-3698731 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030