Detailed information of TA system TA178194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 297494..298002
• Replicon: chromosome
• Accession: NZ_CP063424
• Organism: Actinobacillus pleuropneumoniae strain APP5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: IOD66_RS01515
• Protein ID: WP_009875288.1
• Coordinates: 297494..297754 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A3N1S6
• Locus tag: IOD66_RS01520
• Protein ID: WP_005605062.1
• Coordinates: 297751..298002 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOD66_RS01475 (IOD66_01490)	292599..293372	+	774	WP_009875285.1	DNA polymerase III subunit epsilon	-
IOD66_RS01490 (IOD66_01505)	293731..294123	-	393	WP_005620423.1	SufE family protein	-
IOD66_RS01495 (IOD66_01510)	294245..294835	+	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
IOD66_RS01500 (IOD66_01515)	294835..296094	+	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
IOD66_RS01505 (IOD66_01520)	296188..297168	+	981	WP_005608502.1	tetraacyldisaccharide 4'-kinase	-
IOD66_RS01510 (IOD66_01525)	297170..297337	+	168	WP_009875287.1	Trm112 family protein	-
IOD66_RS01515 (IOD66_01530)	297494..297754	-	261	WP_009875288.1	Txe/YoeB family addiction module toxin	Toxin
IOD66_RS01520 (IOD66_01535)	297751..298002	-	252	WP_005605062.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
IOD66_RS01530 (IOD66_01545)	298340..299779	-	1440	WP_009875289.1	glutamate--tRNA ligase	-
IOD66_RS01555 (IOD66_01570)	300683..301864	+	1182	WP_009875290.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
IOD66_RS01560 (IOD66_01575)	301918..302466	+	549	WP_011848557.1	type IV pilus biogenesis/stability protein PilW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10452.00 Da
• Isoelectric Point: 8.0109

>T178194 WP_009875288.1 NZ_CP063424:c297754-297494 [Actinobacillus pleuropneumoniae]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKPEPLKFNLSGFWSRRINEEHRLIYTVEDETILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T178194 NZ_CP083877:3443872-3444090 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9320.62 Da
• Isoelectric Point: 4.5659

>AT178194 WP_005605062.1 NZ_CP063424:c298002-297751 [Actinobacillus pleuropneumoniae]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYSSLEETAYLMRSPANAQRLLDSMAELKQGKGIERE
LIE

Nucleotide

• Length: 252 bp

>AT178194 NZ_CP083877:3443472-3443846 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A3N1S6